Abcam, ab234042, Uricase Assay Kit (Fluorometric)

Size: 100Test
Uricase Assay Kit (Fluorometric) ab234042 provides a quick and easy method for the measurement of uricase activity in a wide variety of samples. Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
Detection method:Fluorescent,
Sample types:Tissue Extracts,
Assay type:Enzyme activity,
Sensitivity:= 10 µU,
Assay time:1h,
Assay Platform:Microplate reader

Product details:
Uricase Assay Kit (Fluorometric) (ab234042) provides a quick and easy method for the measurement of uricase activity in a wide variety of samples.
In this assay, uricase oxidizes uric acid forming a product that reacts with the probe, thus generating a fluorescence signal (Ex/Em= 535/587nm). The generated fluorescence signal is directly proportional to the level of uricase activity in the sample. The kit provides a sensitive, high throughput and adaptable assay that can measure uricase activity as low as 10 uU in biological samples.
Other Notes
This product was previously called K734 Biovision Uricase Activity Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.
Uricase (factor-independent urate hydroxylase, urate oxidase (UO); EC 1.7.3.3) is an enzyme involved in the uric acid metabolism. Uric acid is the end product of purine metabolism, and high levels of uric acid in blood causes gout. Uricase is present in a wide range of mammals but absent from human beings.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Uricase also known as urate oxidase is an enzyme with a mass of approximately 37 kDa. This enzyme catalyzes the conversion of uric acid to allantoin carbon dioxide and hydrogen peroxide facilitating the degradation of uric acid. Uricase is found in the liver of many animals including primates and some rodents. However humans and great apes lack functional uricase due to a genetic mutation that occurred during evolution which might contribute to higher uric acid levels in their systems.
Biological function summary
In species with active uricase the enzyme plays a role in regulating uric acid levels by breaking down excess uric acid which is a byproduct of purine metabolism. Uricase operates as a homotetramer complex where four identical subunits work together to execute its enzymatic function efficiently. The enzyme is key in preventing the accumulation of uric acid which could otherwise form crystals leading to potential health complications.
Pathways
Uricase functions within the purine catabolic pathway. The enzyme ensures the smooth degradation of purine with its activity linking it to various other enzymes involved in the breakdown of nucleotides. This enzyme's activity is associated with other proteins like xanthine oxidase and adenine deaminase which also contribute to purine metabolism. Although humans lack uricase these pathways are important for managing purine levels and their end-products impacting overall metabolic balance.
The absence of uricase in humans plays a role in conditions like gout and hyperuricemia which are characterized by elevated uric acid levels and crystal formation in joints and tissues. Uricase's deficiency means humans rely more on related proteins like xanthine oxidase for purine metabolism potentially leading to less efficient processing of uric acid. Therapies often involve uricase from other species or drugs like allopurinol which inhibit xanthine oxidase to manage these diseases by reducing uric acid concentration in the body.