Abcam, ab234047, Lysosome Isolation Kit

Size: 50Test
Lysosome isolation kit (ab234047) provides a procedure for isolating enriched or purified lysosomal fractions from tissues and cultured cells by differential centrifugation, followed by density gradient centrifugation. - Use lysosomal fractions for downstream applications including activity assays, SDS-PAGE, Western blot Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
Key facts
Sample types:Tissue, Suspension cells, Adherent cells

Product details:
Lysosome Isolation Kit (ab234047) provides a procedure for isolating an enriched or purified lysosomal fraction from animal tissues and cultured cells by differential centrifugation followed by density gradient centrifugation.
Lysosome Isolation protocol summary
- Isolate and homogenize cells or tissue
- Centrifuge homogenate and keep supernatent
- Carefully add supernatent to discontinuous density gradient
- Remove band containing enriched lysosome fraction
- Use for downstream applications e.g. activity assays, SDS-PAGE, Western blot
This product is manufactured by BioVision, an Abcam company and was previously called K235 Lysosome Purification Kit. K235-50 is the same size as the 50 test size of ab234047.
Lysosomes are membrane-bound cell organelles found in most animal cells (except red blood cells). Lysosomes are spherical vesicles containing hydrolytic enzymes capable of breaking down different kinds of biomolecules, including proteins, nucleic acids, carbohydrates, lipids, and cellular debris. They are known to contain over 50 different enzymes, which are active under acidic conditions. Thus, lysosomes act as the waste disposal system of the cells by digesting unwanted materials in the cytoplasm.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols