Abcam, ab234087, Anti-ARFGAP1 antibody [1C4E2]

Size: 100µg
Mouse Monoclonal ARFGAP1 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARFGAP1 aa 250 to C-terminus.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1C4E2,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ARFGAP1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8N6T3

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from tissue culture supernatant., Storage buffer-Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARFGAP1 also known as ADP-ribosylation factor GTPase-activating protein 1 operates mechanically to regulate the activity of ARF family proteins by enhancing GTP hydrolysis. Its enzymatic role involves facilitating ARF in their active and inactive states performing critical functions in vesicular trafficking and membrane dynamics. ARFGAP1 has a molecular weight of approximately 50 kDa and shows expression in various tissues where it particularly functions with intracellular membranes linked to the Golgi apparatus.
Biological function summary
ARFGAP1 plays a significant role in intracellular trafficking and its activity forms an essential part of the COPI coat protein complex affecting vesicle formation and transport across the Golgi. This complex collaborates closely with ARFs to maintain cellular cargo trafficking and organelle structure integrity. Efficient functioning of ARFGAP1 is necessary for proper protein transport within the cell reflecting its participation in essential cellular processes.
Pathways
Several significant cellular processes involve ARFGAP1 through its contribution to the vesicular transport and endocytosis pathways. Within these pathways ARFGAP1 coordinates with proteins like ARF1 which is important for vesicle budding and cargo selection. It ensures the accurate conveyance and distribution of proteins and lipids impacting numerous cellular mechanisms and their regulatory controls.
Improper ARFGAP1 function associates with conditions like cancer and neurological disorders. Its dysregulation can affect cellular transport processes thereby influencing disease progression. In cancer altered ARFGAP1 activity interacts with proteins such as ARF1 potentially contributing to abnormal cell division and metastasis. Its involvement in neurological disorders is linked to disruptions in membrane trafficking affecting neuron communication and function.