Abcam, ab234260, Anti-Surfactant protein D/SP-D antibody [EPR21774-153] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Surfactant protein D/SP-D antibody. Carrier free. Suitable for IHC-P, WB, IHC-Fr and reacts with Rat, Recombinant fragment, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21774-153,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:IHC-Fr, IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The Mouse species recommendation is based on the WB results. We do not guarantee IHC for Mouse.

Product details:
ab234260 is the carrier-free version of
ab220422
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Surfactant protein D (SP-D) also known as protein D functions as an important component of the innate immune system. It weighs around 43 kDa and is primarily expressed in pulmonary alveoli where lung cells synthesize it. SP-D belongs to the collectin family and has a C-type lectin domain that allows binding to carbohydrate structures on microbial surfaces. This ability enables SP-D to interact directly with various pathogens and infected cells targeting them for clearance. Researchers often study SP-D with 'anti-surfactant' antibodies using methods like the ELISA protocol including versions like ELISA Sp and ELISA Denise.
Biological function summary
SP-D contributes to host defense by enhancing the uptake and elimination of pathogens by immune cells such as macrophages. Part of a larger protein complex SP-D can opsonize bacteria and viruses facilitating phagocytosis. Its role extends to modulating inflammation by affecting the production of pro-inflammatory cytokines. By maintaining homeostasis in surfactant composition it indirectly supports lung function and protects against lung injury. SP-D’s function involves communication and transmission of signals within the immune system making it integral to pulmonary immune processes.
Pathways
Surfactant protein D is involved in the regulation of the complement and coagulation cascades as well as the pathogen recognition pathway. It is closely related to other collectins such as surfactant protein A (SP-A) which share similar mechanisms in the immune response. SP-D's interaction within these pathways highlights its role in identifying and neutralizing pathogens. Through its connections with related proteins SP-D plays an important part in modulating immune responses ensuring effective pathogen elimination without excessive inflammation.
SP-D has been linked with respiratory diseases such as chronic obstructive pulmonary disease (COPD) and pulmonary fibrosis. These conditions may involve impaired SP-D function or expression levels potentially due to genetic factors or environmental influences. SP-D also interacts with proteins like SP-A in these diseases participating in lung immune responses. Disrupted regulation of SP-D can contribute to chronic inflammatory states in the lungs exacerbating disease progression and leading to compromised lung function. Understanding SP-D's role in these contexts offers potential therapeutic insights for targeting pathological inflammation.