Abcam, ab234957, Anti-DNA:RNA hybrid antibody [S9.6]

Size: 20µg / 100µg / 1mg
Anti-DNA:RNA hybrid antibody [S9.6] (ab234957) is a mouse monoclonal antibody detecting DNA:RNA hybrid in IP, Dot Blot .
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:S9.6,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Applications:IP, DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The S9.6 monoclonal recognizes DNA-RNA hybrids (also known as R-loops) and does not bind to single or double stranded DNA (PubMed IDs: 2422282, 16614443). The antibody has high affinity for DNA-RNA hybrids but also binds RNA-RNA hybrids that are AU-rich (PubMed ID: 23784994). The specificity of the antibody appears to be determined by a combination of sequence and structural dependency since R-loop sequence affects binding affinity (PubMed ID: 28594954). It is important to use controls such as RNase A treatment in light of the cross-reactivity with double stranded RNA. Please see our DNA-RNA-IP (DRIP) protocol for further information. We do not recommend using this antibody for imaging applications such as ICC-IF, IHC or Flow due to the cross-reactivity with double stranded RNA.

Product details:
The pCALM3_2 vector used to generate R-loops was kindly provided by Prof. Frederic Chedin at UC Davis. The pCALM3_2 plasmid carries a 789bp fragment of the human CALM3 region that forms R-loops (PubMed ID: 31053798) cloned between T3 and T7 RNA polymerase promoter sequences. Transcription by T3 RNA polymerase leads to R-loop formation. Transcription by T7 RNA polymerase does not allow R-loop formation.
Our DNA-RNA immunoprecipitation (DRIP) protocol is linked
Product Specifications
Anti-DNA:RNA hybrid antibody [S9.6] (ab234957) is a mouse recombinant monoclonal antibody and is validated for use in Dot blot, IP.
Anti-DNA:RNA hybrid antibody [S9.6] (ab234957) specifically detects DNA:RNA hybrid (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µg and 1 mg selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-DNA:RNA hybrid antibody [S9.6] (ab234957) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle