Abcam, ab235081, Human KIM1 ELISA Kit (TIM-1)

Size: 1 x 96Tests
Human KIM1 ELISA Kit (TIM-1) is a Sandwich (quantitative) ELISA for the measurement of Human KIM1 (TIM-1) in Human in Biofluids samples.
Key facts
Detection method:Colorimetric,
Sample types:Urine,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.279 pg/mL,
Range:7.813 - 500 pg/mL,
Assay time:2h,
Assay Platform:Microplate

Product details:
The human KIM1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is a complete kit for the quantitative measurement of KIM1 protein in urine.
The ELISA kit is ultra sensitive, reliably measuring as little as 1.2 pg/ml of Kim1. This enables the use of reduced input sample and results in reduced matrix interference. It is high throughput with results in <2 hours from up to 38 samples in duplicate. The kit uses a monoclonal antibody to KIM-1 immobilized on a microtiter plate to bind the KIM-1 in the standards or sample. After a short incubation the excess sample or standard is washed out and a biotinylated monoclonal antibody to KIM-1 is added. This antibody binds to the KIM-1 captured on the plate. After a short incubation the excess antibody is washed out and Streptavidin conjugated to Horseradish peroxidase is added, which binds to the biotin on the monoclonal antibody. The plate is then incubated. Once the incubation is complete, excess conjugate is washed out and TMB substrate solution is added. An HRP-catalyzed reaction generates a blue color in the solution. Stop solution is added to stop the substrate reaction. The resulting yellow color is read at 450 nm. The amount of signal is directly proportional to the level of KIM-1 in the sample.
Kim1 is an early biomarker for kidney injury or disease. It is the most highly upregulated protein in the proximal tubule of the injured kidney. It exists in very low levels in normal kidneys but when upregulated during injury, it is detectable in urine in a wide variety of human diseases. KIM-1 is a potential biomarker for renal injury, which would suggest it has great importance in various kidney diseases and disorders such as chronic kidney disease, as well as acute tubular necrosis and acute kidney failure.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TIM 1 also known as T-cell immunoglobulin and mucin domain 1 is a cell surface protein with a molecular mass of approximately 70 to 80 kDa. It is expressed predominantly in activated T cells and some epithelial cells. TIM 1 plays an important role in immune regulation and can be found at the surface of cells in various tissues including the liver and kidney. This protein can interact with other components which can influence immune response in the host.
Biological function summary
The TIM 1 protein functions as a receptor involved in T cell activity modulation and innate immune response regulation. It is known to bind phosphatidylserine on apoptotic cells which fosters communication between dying cells and phagocytes. TIM 1 does not typically exist as part of a larger complex but its interactions with other molecules is important for its role in the immune system. The protein’s activity influences cytokine production and this can affect inflammation processes.
Pathways
TIM 1 is involved in key immune response pathways like the signaling pathways of T cells and the broader adaptive immune response. This protein has been linked with the enhancement of Th2 cytokine production impacting allergic response signaling. It interacts with related proteins like TIM 3 and TIM 4 which together contribute to the fine-tuning of immune responses. Understanding TIM 1 within these pathways helps in grasping its regulatory potential in T-cell mediated immunity.
TIM 1 is implicated in conditions such as asthma and systemic lupus erythematosus (SLE). In asthma improperly regulated Th2 immune responses involve TIM 1 which can exacerbate allergic inflammations. This protein also plays a role in the pathogenesis of SLE a systemic autoimmune disease where TIM 1's regulation of immune tolerance and inflammation is critical. TIM 1’s involvement with other proteins like IL-4 and IL-13 in these disorders further highlights its significance in immune dysregulation processes.