Abcam, ab235900, Phagocytosis Assay Kit (Green E.coli)

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Phagocytosis Assay Kit (Green E. coli) (ab235900) uses heat-killed E. coli particles, that are fluorescently labeled with a green dye, to detect and quantify in vitro phagocytosis by fluorescent microscope, fluorometric plate reader or flow cytometry. - Ex/Em 490/520 nm - Validated as macrophage phagocytosis assay. - includes labeled E.coli and assay buffer. Also includes quenching solution for readout on microscope / plate reader.
Key facts
Detection method:Fluorescent,
Sample types:Suspension cells, Adherent cells,
Assay Platform:Microplate reader, Fluor. microscope, Flow cyt.

Product details:
Phagocytosis Assay Kit (Green
E. coli
) (ab235900) uses heat-killed, fluorescently pre-labeled
E. coli
particles as a tool for rapid and accurate detection and quantification of in vitro phagocytosis by fluorescent microscope, spectrophotometer or flow cytometry. The kit provides a robust screening system for activators and/or inhibitors of phagocytosis and Toll-like receptor (TLR) ligands.
Phagocytosis assay protocol summary
- add Green E. coli reagent to cells, and incubate for 2-3 hours
- harvest cells by centrifugation, remove supernatant, and resuspend cells in Assay Buffer; repeat wash step 3 times
- for fluorescent microscope or fluorometric plate reader analysis, use Quenching Solution
- analyze cells with flow cytometer, fluorescent microscope, or fluorometric plate reader
Related phagocytosis assays
Phagocytosis Assay Kit (Red E. coli)
ab235901
Phagocytosis Assay Kit (Green Zymosan)
ab234053
Phagocytosis Assay Kit (Red Zymosan)
ab234054
Phagocytosis Assay Kit (Zymosan, Colorimetric)
ab211156
Other Notes
This product was previously called K963 Biovision EZCell™ Phagocytosis Assay Kit (Green E. coli). Biovision was acquired by Abcam in 2021.
Phagocytosis in mammals serves as an important first line defense mechanism against invading pathogens. It is also essential for continuous clearance of dying cells, tissue remodeling, and acquisition of nutrients for some cells. Phagocytosis is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides.
E. coli
and other bacterial strains are often used as a pathogen in phagocytosis assays.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols