Abcam, ab236088, Anti-Glucuronic Acid Epimerase/GLCE antibody

Size: 100µg / 100µL
Rabbit Polyclonal Glucuronic Acid Epimerase/GLCE antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GLCE aa 1 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human GLCE aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.O94923

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glucuronic Acid Epimerase also known as GLCE is an enzyme that epimerizes D-glucuronic acid to L-iduronic acid in the biosynthesis of glycosaminoglycans like heparan sulfate. This enzyme has a molecular mass of approximately 39 kDa. It is mainly expressed in human tissues such as liver kidney and placenta. Expression levels might vary slightly depending on the specific tissue but these are areas where significant enzyme activity is observed.
Biological function summary
Glucuronic Acid Epimerase plays an important role in the modification of glycosaminoglycans by changing the stereochemistry of uronic acid residues. This ability to alter sugar components is important for creating diverse structural and functional variations in heparan sulfate chains. Though not part of a larger protein complex GLCE's activity significantly affects the structural integrity and function of extracellular matrix components.
Pathways
Glucuronic Acid Epimerase is involved in the heparan sulfate biosynthesis pathway which is essential for cell signaling and interaction. The enzyme coordinates with other biosynthetic enzymes like N-deacetylase/N-sulfotransferase (NDST) to generate unique heparan sulfate structures. These structures mediate interactions with growth factors within the heparan sulfate biosynthesis and modification pathway.
Altered Glucuronic Acid Epimerase activity might influence pathological conditions such as cancer and cardiovascular diseases. Aberrant heparan sulfate structures linked to irregular GLCE function can contribute to tumor growth by affecting cell proliferation and angiogenesis. Additionally disorders like Ehlers-Danlos syndrome which also involves abnormal glycosaminoglycan metabolism might relate to GLCE activity. Interactions with proteins such as fibroblast growth factors (FGF) might influence these disease processes.