Abcam, ab236127, Anti-Rab9 antibody [EPR13272] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Rab9 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13272,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab236127 is the carrier-free version of
ab179815
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Rab9 also known as Ras-related protein Rab-9A is a small GTPase and part of the Rab family with a mass of around 23 kDa. This protein mainly resides in the trans-Golgi network and late endosomes. Rab9 plays an important role in the transport of mannose 6-phosphate receptors between late endosomes and the Golgi apparatus facilitating cargo sorting and vesicular trafficking in cells.
Biological function summary
Rab9 coordinates several intracellular trafficking processes aiding in the regulation of membrane identity. It associates with other effectors such as tail-interacting protein (TIP47) to form complexes on the endosomal surface. These complexes help in vesicular transport ensuring efficient recycling of receptors. Rab9 impacts endosome maturation and transport making it an essential endosome marker.
Pathways
Rab9 influences and is involved in the regulation of the endocytic and secretory pathways. It interacts with proteins like Rab7 another GTPase to manage late endosome and lysosome transport. These interactions underline Rab9's functionalities in intracellular trafficking and recycling processes relying on cooperative interactions with other Rabs and their effectors.
Rab9 plays a role in conditions like Niemann-Pick disease type C and some neurological disorders. Disruption in Rab9 function may affect lipid trafficking and lead to accumulation of cholesterol in late endosomes. This links Rab9 indirectly to proteins such as NPC1 which is known to be implicated in cholesterol homeostasis. Understanding Rab9's involvement can help inform therapeutic strategies targeting these pathways in related diseases.