Abcam, ab236143, Anti-NTH1 antibody [EPR15930] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal NTH1 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15930,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:WB, ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab236143 is the carrier-free version of
ab191413
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NTH1 protein also known as endonuclease III-like protein 1 functions as a DNA glycosylase involved in base excision repair. It plays a role in the repair of oxidative DNA damage. NTH1 has a molecular mass of approximately 33 kDa. This protein is located in various tissues throughout the human body but it shows higher expression in the testes heart and skeletal muscle. These expression patterns suggest its significance in oxidative stress response across different body systems.
Biological function summary
NTH1 removes oxidized pyrimidines from damaged DNA as part of its role in cellular repair mechanisms. As a component of the base excision repair pathway it tackles oxidative lesions to prevent mutation accumulation. It does not act as a complex with other proteins but operates after being recruited to relevant DNA sites requiring repair. This essential function keeps genomic integrity intact and allows for regular cellular processes.
Pathways
NTH1 plays an important role within the base excision repair and DNA damage response pathways. It cooperates with proteins such as APE1 which prepares the site for subsequent repair steps ensuring proper DNA maintenance. NTH1’s involvement in base excision repair specifically addresses small-scale oxidative damage highlighting its role in safeguarding genetic information. These pathways are fundamental for normal cell function and response to environmental stressors.
NTH1 has links to conditions involving accumulated DNA damage such as cancer and neurodegenerative diseases. DNA repair deficiency due to NTH1 malfunction can contribute to the onset of such diseases. The protein interacts with partners like OGG1 which also plays a role in oxidative DNA lesion recognition and repair to manage DNA stability. Understanding the dysfunction of NTH1 in these contexts helps in exploring potential therapeutic targets and diagnostic markers for related disorders.