Abcam, ab236151, Anti-MCM6 antibody [EPR17686] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MCM6 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR17686,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab236151 is the carrier-free version of
ab201683
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target known as MCM6 or Minichromosome Maintenance Complex Component 6 is a part of a group of proteins providing essential functions during DNA replication initiation. MCM6 is a part of the hexameric MCM2-7 complex which operates as a helicase that unwinds DNA to facilitate fork progression. This protein has an approximate molecular weight of 92 kDa. MCM6 expresses in dividing cells across different tissues particularly in cells with high proliferative rates like those in the bone marrow and gastrointestinal tract.
Biological function summary
The functions of MCM6 contribute to the precise control of DNA replication and overall cell cycle regulation. The protein forms a part of the MCM2-7 complex playing a significant role in the S phase to ensure DNA replication occurs at the right time. Additionally the MCM complex acts as a primary licensing factor for replication origins preventing re-replication. MCM6 also interacts with other proteins like CDC45 and GINS to form the CMG complex a critical component for replication fork stability and duplication.
Pathways
MCM6 contributes significantly within the DNA replication and cell cycle pathways. As a component of the MCM2-7 complex it supports the unwinding of DNA which is a prerequisite for replication to initiate. MCM6 is related to proteins like CDC6 and ORC which are integral to the assembly of the pre-replication complex facilitating the initiation of DNA synthesis. This integration into core cellular mechanisms highlights its importance in genomic stability.
Mutations or deregulation of MCM6 have links to cancerous growths and genomic instability disorders. The aberrant expression of MCM6 is often observed in cancers such as colorectal and breast cancer where it contributes to unchecked cellular proliferation. MCM6 correlates with oncogenic proteins like MYC which can amplify abnormal cell cycle progression. Understanding the mechanistic contributions of MCM6 in these conditions assists in developing therapeutic strategies targeting these pathways.