Abcam, ab236285, Anti-GPCR GPR40 antibody

Size: 100µL
Rabbit Polyclonal GPCR GPR40 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FFAR1 aa 100-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human FFAR1 aa 100-200. The exact immunogen used to generate this antibody is proprietary information.O14842

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GPR40 also known as Free Fatty Acid Receptor 1 (FFAR1) is a G-protein-coupled receptor (GPCR) that primarily binds long-chain free fatty acids. It has a molecular mass of approximately 35 kDa. This receptor is mainly expressed in the pancreatic beta cells and to a lesser extent in the brain gastrointestinal tract and immune cells. GPR40 plays a significant role in mediating the effects of fatty acids on various cells by acting as a signaling receptor in these tissues.
Biological function summary
GPR40 is integral to the regulation of glucose homeostasis. This receptor functions as a part of a complex that facilitates the enhancement of insulin secretion in response to elevated blood glucose levels. When activated GPR40 modulates the release of insulin from pancreatic beta cells contributing significantly to glucose metabolism. This function places GPR40 as an important player in maintaining metabolic balance especially in response to dietary fatty acids.
Pathways
GPR40's role in glucose homeostasis places it within the insulin signaling and fatty acid metabolism pathways. It interacts with proteins like Gq and phospholipase C which transmit signals leading to insulin secretion. These pathways highlight the functional role GPR40 plays in linking nutrient sensing to hormone secretion which positions it alongside other receptors that mediate metabolic processes such as glucose-dependent insulinotropic polypeptide (GIP).
GPR40 has a strong connection to type 2 diabetes and metabolic syndromes. Alterations in the expression or function of GPR40 can influence the insulin secretion capability of pancreatic beta cells potentially leading to insulin resistance and hyperglycemia. Additionally GPR40 has been studied in relation to obesity with changes in its signaling suggested to impact energy balance. Its association with other proteins like incretin hormones underlines its relevance in these metabolic disorders making it a target of interest for therapeutic intervention.