Abcam, ab236397, Anti-MCEE antibody [OTI1B5]

Size: 100µL
Mouse Monoclonal MCEE antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MCEE.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OTI1B5,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human MCEE.Q96PE7

Product details:
Clone OTI1B5 (formerly 1B5).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from cell culture supernatant., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MCEE also known as methylmalonyl-CoA epimerase is an important enzyme with a mass of approximately 17 kDa. This enzyme is found in the mitochondria and expressed widely across different tissue types. MCEE plays a mechanical role in the conversion of D-methylmalonyl-CoA to L-methylmalonyl-CoA a necessary step in the metabolism of certain fatty acids and amino acids. The proper functioning of this enzyme is essential for efficient metabolic pathways and energy conversion in cells.
Biological function summary
Methylmalonyl-CoA epimerase contributes to the metabolism by facilitating the reversible epimerization process of methylmalonyl-CoA. MCEE is part of an enzymatic complex alongside methylmalonyl-CoA mutase which further processes the epimerized product to succinyl-CoA. This pathway is important for the breakdown and utilization of odd-chain fatty acids and certain branched-chain amino acids feeding into the citric acid cycle which is a critical pathway for cellular energy production.
Pathways
Methylmalonyl-CoA epimerase finds its place in the catabolism of β-oxidation and is directly involved in the propionic acid pathway. This positions MCEE as an integral part in facilitating the conversion of lipids and branched-chain amino acids into energy. It works closely with methylmalonyl-CoA mutase which acts downstream linking MCEE to broader metabolic processes and ensuring the continuation of energy supply within the metabolic network. This relationship is important for maintaining cellular homeostasis particularly in energy-demanding tissues.
Disruptions in the function of methylmalonyl-CoA epimerase can relate to metabolic disorders like methylmalonic acidemia. This condition arises from the accumulation of methylmalonic acid due to ineffective conversion because of enzyme defects. MCEE shows connection with proteins like methylmalonyl-CoA mutase which when dysfunctional exacerbates the metabolic imbalance. Additionally MCEE defects may influence disorders related to oxidative stress as improper metabolite handling affects energy production and cellular health.