Abcam, ab236523, Anti-TOPBP 1 antibody [OTI5C12]

Size: 100µL
Mouse Monoclonal TopBP1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TOPBP1 aa 850-1150.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OTI5C12,
Isotype:IgG2a,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human TOPBP1 aa 850-1150. The exact immunogen used to generate this antibody is proprietary information.Q92547

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from cell culture supernatant., Storage buffer-pH: 7.3Preservative: 0.02% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TOPBP1 also known as DNA topoisomerase II-binding protein 1 plays a central role in DNA replication and repair. It has a molecular weight of approximately 180 kDa. TOPBP1 functions as a scaffold protein with a multidomain structure allowing it to interact with various proteins involved in these processes. It is expressed in several tissues including those undergoing high rates of cell division like hematopoietic and epithelial tissues. Its presence in these tissues indicates its involvement in managing and distributing genomic information.
Biological function summary
TOPBP1 is fundamental for maintaining genome integrity. It acts as a scaffold within the cell assembling and recruiting other proteins necessary for DNA replication and checkpoint signaling complexes. As part of the ATR-Chk1 checkpoint pathway TOPBP1 is essential in responding to DNA damage by activating ATR a critical kinase involved in the DNA damage response. Through these interactions TOPBP1 ensures that the cell appropriately halts the cell cycle to allow DNA repair or to trigger apoptosis if the damage is beyond repair.
Pathways
TOPBP1 plays an important role in the DNA damage response and the cell cycle control pathways. Within the DNA replication stress response it is important for the ATR signaling pathway where it works alongside proteins like RAD9-RAD1-HUS1 and Claspin to amplify damage signals. TOPBP1 coordinates the replication restart after fork stalling interacting with other proteins like BRCA1 to stabilize replication forks and prevent chromosomal abnormalities.
Research links TOPBP1 to cancer development and progression. Its role in maintaining genomic stability implicates it in various cancers when mutated or dysregulated. Aberrant TOPBP1 function can lead to unchecked cell division and tumorigenesis. Additionally mutations or changes in TOPBP1 levels are connected to Ataxia telangiectasia a disorder characterized by increased sensitivity to ionizing radiation and predisposition to cancer. In this context TOPBP1 interacts with proteins such as ATM which is another key player in the DNA damage response pathway.