Abcam, ab236986, Anti-Mkln1 antibody

Size: 100µL
Rabbit Polyclonal MKLN1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MKLN1 aa 450-650.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:IHC-P, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MKLN1 aa 450-650. The exact immunogen used to generate this antibody is proprietary information.Q9UL63

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity >95%, Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Mkl1 also known as megakaryoblastic leukemia (translocation) 1 is a protein encoded by the MKL1 gene. The mass of Mkl1 approximately measures around 130 kDa. This protein is expressed in multiple tissues but it exhibits higher expression in the heart and skeletal muscle. Mkl1 localizes mainly in the nucleus where it functions as a coactivator of serum response factor (SRF) playing an important role in actin dynamics and transcriptional regulation.
Biological function summary
Mkl1 coordinates transcriptional programs required for cell differentiation and cytoskeletal organization. As a component of the MRTF-SRF complex Mkl1 regulates genes involved in actin cytoskeleton remodeling. This function impacts cell migration adhesion and morphology influenced by external signals. Additionally it plays a role in the regulation of smooth muscle and cardiac-specific gene expression which highlights its importance in muscle physiology.
Pathways
Mkl1 serves an important function in the RhoA-actin signaling pathway. This pathway controls the organization of the actin cytoskeleton and is connected with several cellular functions including cell shape and motility. Mkl1 interacts closely with SRF as well as other proteins like RhoA GTPase which are vital for transmitting signals that promote actin polymerization and cell movement. This pathway integration contributes to understanding how extracellular cues translate into cytoskeletal rearrangements.
Mkl1 has significant connections to megakaryoblastic leukemia and cardiac hypertrophy. In leukemia a chromosomal translocation involving Mkl1 and another protein MOZ leads to the production of an oncogenic fusion protein that causes dysregulation of normal gene expression contributing to malignancy. Additionally upregulated Mkl1 expression links to cardiac hypertrophy where it facilitates pathological gene expression in heart muscle cells. The role of Mkl1 in these disorders highlights its significance in disease progression and susceptibility.