Abcam, ab237521, Anti-HYPE antibody

Size: 100µL
Rabbit Polyclonal HYPE antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human FICD aa 50-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human FICD aa 50-200. The exact immunogen used to generate this antibody is proprietary information.Q9BVA6

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity greater than 95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HYPE also known as RHBDF1 is an endoplasmic reticulum protein that functions as a membrane protein involved in protein regulation and trafficking. It has an approximate mass of 38 kDa. HYPE is expressed widely within various tissues but shows significant levels in cells of the liver and kidney. This protein plays a role in multiple cellular processes reflecting its versatile functionality.
Biological function summary
This protein impacts endoplasmic reticulum stress responses and protein homeostasis. It does not seem to form part of a larger complex but operates independently to regulate the degradation of specific proteins aiding in maintaining cellular balance. HYPE's actions contribute to preventing protein aggregation that could disrupt cellular function.
Pathways
HYPE participates in the unfolded protein response (UPR) and the ER-associated degradation (ERAD) pathway. It acts alongside proteins involved in protein folding and trafficking such as BiP and calnexin to ensure synthesis and processing occur efficiently. These pathways highlight HYPE’s interaction with mechanisms that manage protein quality control.
This protein has connections to liver fibrosis and kidney dysfunction. Its regulation of protein folding is significant in these diseases where improper protein management can lead to cellular damage. Researchers have identified its interaction with proteins like TGF-beta in the context of fibrosis linking its importance to disease progression and providing potential therapeutic targets.