Abcam, ab237542, Anti-DEPDC1B antibody

Size: 100µL
Rabbit Polyclonal DEPDC1B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DEPDC1B aa 300 to C-terminus.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human DEPDC1B aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.Q8WUY9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity greater than 95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DEP domain-containing protein 1B (DEPDC1B) is a protein that plays a role in the regulation of cell cycle and cytoskeletal dynamics. Also known as DEP.13 this protein has a molecular mass of approximately 70 kDa. DEPDC1B is expressed in various tissues with notably higher expression levels in testis and prostate tissues. This protein is characterized by the presence of a RhoGAP domain which suggests its involvement in modulating Rho family GTPases influencing cytoskeletal organization.
Biological function summary
DEPDC1B is involved in cell migration and mitosis. It operates as part of a larger protein complex that interacts with other regulatory proteins. Its interaction with RhoA and Cdc42 highlights its role in promoting changes in cell morphology and motility. DEPDC1B contributes to cell cycle progression especially during the transition from G1 to S phase indicating its importance in cell proliferation and growth.
Pathways
DEPDC1B is significant in the Rho GTPase signaling pathway and the cell cycle regulation pathway. Its function in these pathways includes co-regulating the activity of proteins like ROCK and MLK which are pivotal for cytoskeletal rearrangement and cell division. DEPDC1B's modulation of these pathways highlights its role in maintaining cellular architecture and ensuring proper cell cycle checkpoints.
Dysfunctions in DEPDC1B have been associated with cancer and neurological disorders. Studies show altered expression of DEPDC1B in certain cancers including prostate cancer linking it to tumor progression and metastasis through its interaction with proteins like RhoA. Additionally abnormalities in its activity are implicated in neurodevelopmental disorders connecting it to proteins influencing neuronal signaling pathways.