Abcam, ab237709, Anti-CD8 alpha antibody [CAL66]

Size: 20µL / 100µL / 1mL
Anti-CD8 alpha antibody [CAL66] (ab237709) is a rabbit monoclonal antibody detecting CD8 alpha in Flow Cytometry, IP, IHC-P, ICC/IF, mIHC . Suitable for Human, Rat . - Multiplex IHC validated on the Leica BOND® MAX using Opal reagents - Biophysical QC for unrivalled batch-batch consistency - Over 40 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:CAL66,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human, Cat, Dog, Cow,
Applications:mIHC, IP, ICC/IF, Flow Cyt, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Product Specifications
Anti-CD8 alpha antibody [CAL66] (ab237709) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, ICC/IF, IHC-P, IP, mIHC in human, rat samples.
Anti-CD8 alpha antibody [CAL66] (ab237709) specifically detects CD8 alpha (UniProt ID: P01732; Molecular weight: 24kDa) and is sold in a convenient trial size to enable initial testing (20 μL) and larger sizes for subsequent scaling up experiments (100 μL and 1 mL).
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-CD8 alpha antibody [CAL66] (ab237709) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD8 alpha antibody [CAL66] (ab237709) has been cited over 43 times in peer reviewed journals and is trusted by the scientific community.
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Conjugation-ready, carrier free format available for antibody clone CAL66 -
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Target Information
CD8 alpha is a transmembrane glycoprotein that functions as a co-receptor for the T-cell receptor (TCR), mainly found on cytotoxic T cells. It plays a crucial role in T cell signaling and cytotoxic activity by binding to MHC class I molecules, aiding the immune response against infected or malignant cells. CD8 alpha is associated with several diseases, particularly those involving immune system dysfunction

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purity >99%, Storage buffer-pH: 7.2 - 7.4Preservative: 0.05% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
Biological function summary
The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.
Pathways
CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha's role in adaptive immunity.
CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha's connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.