Abcam, ab23840, FITC Anti-HLA B27 antibody [HLA-ABC-m3]

Size: 100Test
Mouse Monoclonal HLA B antibody - conjugated to FITC. Suitable for Flow Cyt and reacts with Human samples. Cited in 5 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:HLA-ABC-m3,
Isotype:IgG2a,
Conjugation:FITC,
Excitation/Emission:Ex: 495nm, Em: 519nm,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.

Properties and Storage Information:
Form-Liquid, Purification notes-ab23840 is a purified IgG prepared by ion exchange chromatography., Storage buffer-pH: 7.4Preservative: 0.09% Sodium azideConstituents: PBS, 0.2% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HLA-B27 also known as Human Leukocyte Antigen-B27 is a specific allele of the HLA-B gene. This protein is part of the major histocompatibility complex (MHC) class I molecules which play a role in the immune system by presenting peptide antigens to T cells. HLA-B27 has an approximate molecular weight of 44 kDa. It is expressed on the surface of most nucleated cells particularly on immune cells. The allele is well-studied for its association with certain inflammatory diseases.
Biological function summary
HLA-B27 presents peptides derived from intracellular proteins enabling cytotoxic T-lymphocytes to recognize and respond to pathogens or damaged cells. It forms a complex with beta-2 microglobulin and peptide antigens facilitating antigen presentation. The HLA-B27 antigen-presenting complex plays a role in immune surveillance by maintaining immune system recognition of foreign peptides and altered self-peptides.
Pathways
HLA-B27 is integral in the antigen processing and presentation pathway. This pathway involves the proteasome TAP transporters and other MHC class I molecules. The protein interacts with molecules like HLA-A and HLA-C within this pathway which also play similar roles in immune surveillance and response. These interactions ensure an effective immune response against intracellular pathogens.
HLA-B27 shows a strong association with ankylosing spondylitis and other spondyloarthropathies. Ankylosing spondylitis is a form of chronic inflammatory arthritis that affects the spine and large joints. In continued research HLA-B27-positive individuals demonstrate increased susceptibility to these disorders. The mechanisms might involve interactions with specific peptide antigens causing inappropriate immune responses. Related proteins such as anti-HLA-ABC FITC can assist in studying and identifying those cells presenting the HLA-B27 antigen aiding in diagnoses.