Abcam, ab238658, Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free

Size: 100µg
Mouse Monoclonal CDK11B + CD11c antibody. Carrier free. Suitable for IHC-Fr, ICC/IF and reacts with Rat samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:OX42,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:Yes,
Reacts with:Rat,
Applications:IHC-Fr, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab238658 is the carrier-free version of
ab1211
IHC protocol advice:
This antibody is not suitable for IHC on paraffin-embedded samples.
For best results in IHC on frozen tissue, the following may help detection:
araformaldehyde** perfusion fixed samples have worked well for many customers.
2) For non-perfused tissue, either snap freeze or emerse in periodate-lysine-paraformaldehyde (PLP) fixative for 24 hours at 4°C. Reduce the concentration of paraformaldehyde to 0.25-0.5% since this increases the staining intensity for immune cell surface markers (PMID: 7868861).
3) PFA-fixed samples will require cryoprotection by sucrose infiltration.
4) Use OCT (TissueTek) compound for embedding.
5) For snap frozen tissue, fix sections in cold acetone for 10 min. Allow to dry for 10 min at room temperature. Wash with water for 10 min.
6) Do not heat the samples or sections.**
7) During the staining procedure, do not allow the sections to dry out.
Our Technical team (technical@abcam.com) will be happy to provide further information and advice.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD11b and CD11c also known as integrins αM and αX respectively are important proteins in the immune system. CD11b with a molecular mass of approximately 165 kDa and CD11c around 150 kDa form integrin complexes with CD18. These proteins are expressed on various immune cells including macrophages monocytes granulocytes and dendritic cells. Their expression can also be detected in a subset of B and T cells. The antibody against CD11b also termed OX-42 is often used in research to identify immune cells that express this surface protein.
Biological function summary
Integrins αM (CD11b) and αX (CD11c) facilitate key processes such as adhesion and migration of leukocytes. Both CD11b and CD11c pair with CD18 to form the Mac-1 (Macrophage-1 antigen also known as CR3) and p15095 complexes respectively. These complexes participate in both innate and adaptive immune responses by binding to various ligands like ICAM-1 and fibrinogen. CD11b/CD18 (Mac-1) additionally plays an essential role in phagocytosis degranulation and respiratory burst important activities for pathogen clearance.
Pathways
These integrins engage in critical signaling pathways including the NF-kappaB and MAPK pathways. They regulate the inflammatory response and cell survival. CD11b interacts closely with other proteins like ICAM-1 and C3b which are involved in these pathways. CD11c also contributes to similar pathways although it plays a more specialized role in dendritic cells impacting antigen presentation and immune regulation.
CD11b and CD11c have associations with conditions like autoimmune diseases and chronic inflammatory disorders. Both proteins show altered expression levels in diseases such as rheumatoid arthritis and systemic lupus erythematosus. For instance in these diseases the Mac-1 complex (CD11b/CD18) frequently exhibits dysregulation affecting leukocyte adhesion and migration. The expression of CD11c is modified in certain leukemias and lymphomas where it correlates with disease progression and prognosis.