Abcam, ab238862, Anti-PIH1D1 antibody

Size: 100µL
Rabbit Polyclonal PIH1D1 antibody. Suitable for IP, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PIH1D1.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human PIH1D1.Q9NWS0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purity greater than 95%., Storage buffer-pH: 7.4Preservative: 0.03% Proclin 300Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PIH1D1 also known as PIH1 domain containing protein 1 plays a mechanical role in stabilizing multi-protein complexes. It weighs approximately 37 kDa. The protein exists mainly within the cytoplasm and nucleus of cells. PIH1D1 is a critical component of the R2TP complex contributing to its structural integrity and function. The R2TP complex plays a significant role in various cellular processes including transcription and the assembly of complexes like snoRNPs.
Biological function summary
PIH1D1 interacts with other proteins to mediate molecular chaperone activities. It forms part of the R2TP complex which stands for RNA polymerase II-associated protein 2; TP is related to tuberous sclerosis complexes. Together with other proteins such as RPAP3 it assists in assembling large multi-subunit complexes. This function is essential in maintaining normal cellular operations and stress responses facilitating the maturation and stabilization of many other proteins.
Pathways
PIH1D1 involves itself in the molecular chaperone network and mTOR signaling pathway. It interacts primarily with proteins related to the chaperone HSP90 where it helps to fold and stabilize newly synthesized proteins. The contact with mTORC1 complex which connects to growth and metabolic pathways illustrates its significant contributions to cell growth regulation and energy status sensing. PIH1D1’s role ensures that the pathways remain functional and adaptable.
Researchers have linked PIH1D1 to certain types of cancer and neurodevelopmental disorders. Its interaction with the tumor suppressor protein TSC1 suggests relevance in cancer pathogenesis where different mutations can disrupt normal cell growth control. Moreover piH1D1's involvement in the assembly of snoRNPs connects it to neurodevelopmental disorders where correct RNA processing is essential. Changes in its function can lead to significant cellular abnormalities contributing to disease progression.