Abcam, ab239238, Biotin Anti-CD81 antibody [M38]

Size: 100µg
Anti-CD81 antibody [M38] - Biotin conjugated (ab239238) is a mouse monoclonal antibody detecting CD81 in Flow Cytometry . Suitable for Human .
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:M38,
Isotype:IgG1,
Conjugation:Biotin,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing CD81 protein. The exact immunogen used to generate this antibody is proprietary information.P60033

Product details:
What is this antibody validated in?
Biotin Anti-CD81 antibody [M38] (ab239238) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt) in Human samples.
Related products
Antibody clone M38 is also available pre-conjugated to a variety of labels for your convenience – Anti-CD81 Biotin [M38] (ab239238).
Other related products
We have a range of other formats of antibody clone [M38] also available for your convenience:
ab79559
ab200565
, APC -
ab233259
, Biotin - ab239238, FITC -
ab239256

Properties and Storage Information:
Form-Liquid, Purification technique-Size-exclusion chromatography, Purification notes-The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions.The reagent is free of unconjugated biotin., Storage buffer-pH: 7.4Preservative: 0.0975% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Store in the dark

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CD81 also known as TAPA-1 or 26 kDa protein is a member of the tetraspanin family featuring four transmembrane domains. The molecular weight of CD81 is approximately 26-28 kDa. This protein exhibits expression in numerous cell types such as leukocytes endothelial cells and epithelial tissues. CD81 plays a mechanical role by facilitating membrane protein interactions and contributing to cellular processes like adhesion and morphogenesis. Researchers have identified specific isoforms like M38 and labels like 1D6 CHAN in studies involving this target often analyzing CD81 through techniques like Western blot to determine expression levels and molecular weight.
Biological function summary
CD81 interacts with other tetraspanins and forms complexes within the membrane to regulate cellular signaling trafficking and adhesion. It participates in the assembly of larger tetraspanin-enriched microdomains which are important for efficient signaling and functional variety. These complexes modulate cell morphology proliferation and development influencing immune responses and pathogen entry to cells. The presence of CD81 in diverse tissues implies its involvement in a wide range of cellular processes forming essential complexes with proteins like integrins that further engage in tissue repair and immunological defense.
Pathways
CD81 plays significant roles in the immune system and viral entry pathways. It interacts with other proteins like CD9 and CD19 within the immune response pathways regulating lymphocyte activation and differentiation. CD81 is notably a coreceptor in the hepatitis C virus (HCV) entry pathway facilitating viral attachment and fusion into host cells. These interactions illustrate CD81's involvement in modulation of immune cell responses and influence on pathogen infection processes integrating into the complex cellular pathways important for maintaining homeostasis and response to external stimuli.
CD81 has connections to hepatitis C virus (HCV) infections and immunological disorders such as systemic lupus erythematosus (SLE). During HCV infection CD81 serves as an important entry point for the virus interacting with proteins like claudin-1 and scavenger receptor class B type I (SR-BI) enabling viral entry and replication within liver cells. In SLE alterations in CD81 expression can impact autoantibody production and lymphocyte behavior contributing to the disease's pathology. Understanding CD81's role in these diseases provides insight into therapeutic targets and potential interventions for managing infections and autoimmune responses.