Abcam, ab239523, Anti-Cdk8 antibody [EPR21005] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CDK8 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR21005,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab239523 is the carrier-free version of
ab229192
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cdk8 also known as Cyclin-dependent kinase 8 forms an important component of the transcription regulation machinery. It has a molecular mass of about 66 kDa and is widely expressed in various tissues though its abundance can vary. Cdk8 operates largely in the nucleus of cells where it participates in regulating gene expression. Cdk8 acts as a kinase by modifying other proteins through phosphorylation which alters their activity and function.
Biological function summary
Cdk8 plays a critical role in modulating transcriptional responses. It does this by becoming part of the Mediator complex which is essential for transcription regulation by RNA Polymerase II. The Mediator complex where Cdk8 acts as a kinase influences the initiation and regulation of gene expression. Cdk8 phosphorylates several transcription factors and other components affecting their ability to activate or repress transcription.
Pathways
Cyclin-dependent kinase 8 functions prominently in the Wnt/β-catenin signaling and Notch signaling pathways both essential for development and cell differentiation. In the Wnt pathway it interacts closely with proteins like β-catenin to influence gene activation. In the Notch pathway it modulates transcription factors related to cellular differentiation processes. These interactions underline its role as a regulator in carefully managing cellular responses to signaling cues.
Cdk8 has been implicated in cancer and congenital disorders. Its overactivity can lead to uncontrolled cell growth contributing to tumor progression notably in colorectal cancer where it affects genes in the Wnt pathway. Beyond cancer its dysfunction links to congenital heart defects where it affects cardiogenesis-associated proteins such as NOTCH1. Cdk8’s involvement in these pathways highlights its part in both regular development and disease pathogenesis.