Abcam, ab239714, PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase)

Size: 100Test
PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase) (ab239714) provides a quick and easy way for measuring PEPCK activity in various samples.
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Suspension cells, Adherent cells,
Assay Platform:Microplate reader

Product details:
PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase) (ab239714) provides a quick and easy way for measuring PEPCK activity in various samples.
In this assay, Phosphoenolpyruvate Carboxykinase is coupled with a set of enzymes that convert PEP and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and converted generating a colorimetric signal (OD: 570 nm). The color intensity is directly proportional to the amount of active Phosphoenolpyruvate Carboxykinase present in samples.
The assay is simple, sensitive, high-throughput adaptable and can detect less than 10 μU of Phosphoenolpyruvate Carboxykinase activity per sample.
This product is manufactured by BioVision, an Abcam company and was previously called K359 Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric). K359-100 is the same size as the 100 test size of ab239714.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Phosphoenolpyruvate Carboxykinase also known as PEPCK or PEP carboxykinase is an enzyme that catalyzes the conversion of phosphoenolpyruvate to pyruvate in gluconeogenesis. This enzyme has a mass of approximately 67 kDa and is expressed in tissues such as the liver kidney and adipose tissue. The PEPCK reaction plays a role in metabolic regulation by maintaining glucose homeostasis particularly during fasting.
Biological function summary
PEPCK facilitates the metabolic pathway by converting oxaloacetate into phosphoenolpyruvate a critical step in gluconeogenesis. It exists in two forms: cytosolic PEPCK-C and mitochondrial PEPCK-M allowing the enzyme to operate in distinct cellular compartments. It does not form a complex with other proteins but it does have a significant role in enabling the synthesis of glucose from non-carbohydrate sources an essential process in energy metabolism.
Pathways
PEPCK participates in gluconeogenesis and the tricarboxylic acid cycle. It interacts with enzymes such as pyruvate carboxylase which provides oxaloacetate as a substrate in gluconeogenesis. The PEP carboxykinase reaction is central to carbohydrate metabolism linking anabolic and catabolic pathways and ensuring that cellular energy demands are met under varying conditions.
PEPCK has associations with diabetes and obesity where its expression levels influence glucose production and insulin sensitivity. Altered PEPCK activity links to metabolic dysregulation which may contribute to hyperglycemia in diabetes. PEPCK is also related to other metabolic enzymes like glucose-6-phosphatase often studied for their roles in metabolic disorders.