Abcam, ab239844, Anti-PPP1CA + PPP1CB antibody [EP1511Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal PPP1A/PPP1CA antibody. Carrier free. Suitable for IP, ICC/IF, Flow Cyt (Intra), IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EP1511Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, ICC/IF, IP, Flow Cyt (Intra), WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:The immunogen for this antibody is 100% homologous with Human PPP1CA and PPP1CB

Product details:
ab239844 is the carrier-free version of
ab52619
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein targets PPP1CA and PPP1CB also known as protein phosphatase 1 catalytic subunits alpha and beta function as serine/threonine phosphatases. They both play roles in a range of cellular processes. The molecular weight of PPP1CA is approximately 37 kDa while PPP1CB is around 38 kDa. These phosphatases express widely across various tissues including the brain muscle and liver contributing to their involvement in diverse cellular functions. Their distribution suggests a broad range of activities across different physiological systems.
Biological function summary
Protein phosphatase 1 catalytic subunits like PPP1CA and PPP1CB regulate important processes such as cell division and glycogen metabolism. These subunits typically are part of larger multiprotein complexes allowing for specific substrate targeting. By dephosphorylating specific proteins they act as regulatory signals within cellular systems influencing numerous biochemical pathways and responses. In neuronal tissues for example these phosphatases impact learning and memory by regulating synaptic plasticity through dephosphorylation cycles.
Pathways
These phosphatase subunits engage in the MAP kinase and PI3K-Akt signaling pathways. In the MAP kinase pathway they interact with proteins such as ERK helping regulate cell proliferation and differentiation. In the PI3K-Akt pathway they modulate the activity of key components influencing processes like cell survival and growth. The regulatory function of PPP1CA and PPP1CB within these pathways facilitates critical cellular responses to various extracellular signals thereby affecting the overall cellular physiology.
Alterations in PPP1CA and PPP1CB activity have links to several health concerns including cancer and diabetes. In certain cancers abnormal expression or mutation of these phosphatases affects cell cycle control leading to unchecked cell growth. In diabetes PPP1CA and PPP1CB influence glycogen metabolism where their dysregulation contributes to impaired glucose homeostasis. These targets connect to other proteins like GSK-3 in cancer and AKT1 in diabetes highlighting their diverse interactions and potential as targets for therapeutic intervention.