Abcam, ab239879, Anti-Estrogen Related Receptor alpha antibody [EPR46Y] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Estrogen Related Receptor alpha antibody. Carrier free. Suitable for IP, Flow Cyt, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR46Y,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Rat, Mouse,
Applications:WB, IP, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab239879 is the carrier-free version of
ab76228
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Estrogen Related Receptor Alpha (ERRα) also known as NR3B1 is an orphan nuclear receptor with a molecular mass of approximately 51 kDa. It acts as a transcription factor and influences the expression of genes involved in energy metabolism. ERRα is expressed in numerous tissues such as the liver heart kidney and skeletal muscles. It binds to DNA as a monomer and alters specific gene expression activities. This protein lacks an identified ligand which classifies it as an orphan receptor but it shares structural similarities with estrogen receptors allowing the regulation of gene transcription in several physiological contexts.
Biological function summary
Estrogen Related Receptor Alpha regulates cellular energy production processes including mitochondrial biogenesis and oxidative phosphorylation. It plays a role in the biological function of energy homeostasis and increases the expression of genes related to metabolic processes. ERRα operates as part of a transcriptional regulatory network and interacts with other nuclear receptors and coactivators to modulate its activity. It often forms complexes with coactivators such as peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) to exert its effects on mitochondrial function.
Pathways
ERRα is integral to the PGC-1α signaling pathway and the AMPK (AMP-activated protein kinase) signaling pathway. These pathways are important in regulating energy metabolism and adaptive thermogenesis. ERRα interacts with PGC-1α to enhance mitochondrial function and energy expenditure while AMPK activation affects ERRα transcriptional activity and facilitates cellular energy balance. Additionally ERRα cross-talks with other nuclear receptors like PPAR (peroxisome proliferator-activated receptor) family members which are involved in lipid metabolism and glucose homeostasis.
ERRα has implications in metabolic conditions such as diabetes and obesity. The protein links to diabetes through its role in mitochondrial dysfunction and insulin resistance. Obesity-related studies point out how ERRα affects adaptive thermogenesis and lipid metabolism contributing to energy imbalance. ERRα-PPAR interactions highlight its association in metabolic disorders influencing various aspects of metabolism and disease progression. Understanding ERRα function offers therapeutic potential for targeting metabolic diseases.