Abcam, ab240007, Anti-HINT1 antibody [EPR5108] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HINT1 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5108,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240007 is the carrier-free version of
ab124912
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HINT1 also known as Histidine Triad Nucleotide-Binding Protein 1 is a hydrolyzing enzyme with a monomeric mass of approximately 14 kDa. It functions mechanically by catalyzing the phosphoramidate bond in nucleotides which releases free phosphates. HINT1 is part of the family of histidine triad proteins and is expressed broadly across various tissues including the brain heart and testis indicating its potential importance in those areas.
Biological function summary
Within cellular processes HINT1 plays a role as a transcriptional regulator especially influencing the p53 pathway and those involved in stress responses. It forms part of a complex with other proteins guiding cellular responses to DNA damage. This activity strongly suggests HINT1's role in maintaining genomic stability and influencing cell cycle checkpoints.
Pathways
Several important signaling cascades involve HINT1 such as p53 and MAPK pathways. In the context of the p53 pathway HINT1 enhances tumor suppression activities by regulating transcription and apoptosis processes. Through the MAPK pathway it influences cell proliferation and apoptosis. Other proteins like p53 itself and c-Myc closely interact with HINT1 within these signaling pathways coordinating responses to cellular stress and growth signals.
There is a strong association between HINT1 and cancer as well as neuropathic disorders. Its role in tumor suppression suggests that dysfunction or altered expression may contribute to cancer progression particularly cancers related to the lung and colon. Moreover studies indicate a link between HINT1 and schizophrenia where alterations in protein expression can affect neural signaling. Proteins such as p53 and NMDAR which are altered in these disorders connect to HINT1 further displaying its significance in the development and progression of these diseases.