Abcam, ab24011, Anti-Respiratory Syncytial Virus Fusion (F) Glycoprotein antibody [RSV3216 (B016)]

Size: 200µg
Anti-Respiratory Syncytial Virus fusion protein antibody [RSV3216 (B016)] (ab24011) is a mouse monoclonal antibody detecting Respiratory Syncytial Virus fusion protein in Western Blot, Flow Cytometry, IP, ICC/IF, ELISA, EM . - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:RSV3216 (B016),
Isotype:IgG2b,
Carrier free:No,
Reacts with:Respiratory syncytial virus,
Applications:ICC/IF, sELISA, EM, Flow Cyt, IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Virus preparation containing F protein. The exact immunogen used to generate this antibody is proprietary information.P03420,
Specificity:Recognises a Respiratory Syncytial Virus fusion protein (46kD and 22kD s-s linked glycoprotein).

Product details:
What is this antibody validated in?
Anti-Respiratory Syncytial Virus fusion protein antibody [RSV3216 (B016)] (ab24011) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA, Electron microscopy (EM) in Respiratory syncytial virus samples.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.5Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Respiratory Syncytial Virus (RSV) fusion protein also called F protein is critical in the viral entry process. It facilitates the fusion of the viral envelope with the host cell membrane permitting the viral RNA to enter the host cell. The fusion protein is a trimeric glycoprotein expressed on the surface of the RSV virion and has a molecular mass of approximately 64 kDa. The protein undergoes maturation through proteolytic processing which is important for its function. RSV fusion protein is primarily expressed in the respiratory tract during infections.
Biological function summary
The RSV fusion protein plays a pivotal role in initiating infection by mediating the fusion of the virus with the host cell membrane. It forms part of a complex that includes other viral envelope glycoproteins such as the attachment (G) protein facilitating viral binding and fusion. The conformational change in the fusion protein triggers the merging of the viral envelope with the host cell membrane an essential step for viral replication and spread within the host.
Pathways
Fusion protein contributes significantly to the viral infection pathway of RSV. During virus-host cell interaction the fusion protein engages with cell surface receptors like nucleolin part of the viral entry pathway. Additionally the fusion protein's activity is related to the broader class I viral fusion proteins which includes influenza hemagglutinin detailing its fundamental role in viral pathogenesis and immune evasion strategies.
The RSV fusion protein is a major factor in illnesses such as bronchiolitis and pneumonia particularly in young children and immunocompromised patients. The fusion protein's activity is associated with the exacerbation of these respiratory conditions. It also interacts with proteins like the G protein during infection which can influence RSV pathogenicity and the host's immune response. Understanding these interactions helps in the development of therapeutic and preventative strategies against RSV-related diseases.