Abcam, ab240192, Anti-SFT antibody [EPR13000(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SFT antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR13000(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Human,
Applications:IP, Flow Cyt (Intra), WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab240192 is the carrier-free version of
ab176561
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein SFT also known as surfactant protein or SP plays a role in reducing surface tension in the lungs. It's a glycoprotein with a known mass of around 32 kDa. SFT mainly gets expressed in the alveolar type II cells in the lungs where it's secreted into the lung's extracellular lining. Its primary function involves aiding in lung stability and preventing alveolar collapse during respiration.
Biological function summary
Surfactant proteins contribute to innate immune defense and lung homeostasis. SFT is a component of the surfactant complex which includes other proteins and lipids. This protein acts at the air-liquid interface in the alveoli and engages in host defense functions. SFT activates alveolar macrophages and binds to a range of pathogens enhancing their clearance from the respiratory tract. It helps regulate inflammatory responses and maintain the balance of surfactant components in the lungs.
Pathways
SFT plays an integral role in the pulmonary surfactant metabolism pathway and the immune response pathway. In the pulmonary surfactant metabolism pathway it interacts with proteins like ABCA3 and lipids like phosphatidylcholine to ensure proper surfactant composition and recycling. In the immune response pathway SFT partners with complement proteins and defensins supporting pathogen clearance and preventing lung infections.
SFT relates primarily to respiratory distress syndrome (RDS) and pulmonary alveolar proteinosis (PAP). In RDS an insufficient amount or dysfunction of surfactant proteins including SFT leads to alveolar collapse and impaired gas exchange. In PAP the protein's accumulation affects surfactant homeostasis disrupting lung function. These diseases tie SFT to other proteins like GM-CSF and ABCA3 which influence surfactant production and regulation.