Abcam, ab2402, Anti-TopBP1 antibody

Size: 50µL
Rabbit Polyclonal TopBP1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human TOPBP1 aa 1400-1450.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human TOPBP1 aa 1400-1450. The exact immunogen used to generate this antibody is proprietary information.Q92547

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG., Storage buffer-pH: 7 - 8Preservative: 0.1% Sodium azideConstituents: PBS, 1.815% Tris, 1.764% Sodium citrate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle, Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TopBP1 also known as Topoisomerase II Binding Protein 1 is an important regulator in DNA replication and repair processes. This protein has an approximate mass of 180 kDa. It is expressed in various tissues with significant levels in rapidly dividing cells. TopBP1 functions mechanically by interacting with components of the DNA damage response machinery. It binds to double-strand breaks and activates ATR (Ataxia Telangiectasia and Rad3-related protein) signaling which is essential for maintaining genome stability under stress conditions.
Biological function summary
The activities of TopBP1 involve coordination of replication checkpoints and safeguarding the integrity of the replication fork. TopBP1 operates as part of a larger protein complex that senses DNA damage and initiates repair processes. It facilitates the recruitment of other repair proteins to sites of damage ensuring that replication proceeds only when the DNA is adequately repaired. It functions closely with proteins like RAD9 RAD1 and HUS1 which sense DNA damage and engage repair pathways.
Pathways
TopBP1 plays an important role in the DNA damage response and cell cycle regulation pathways. The protein is primarily involved in the ATR-Chk1 pathway and plays a significant role in the replication stress response. Within these pathways TopBP1 associates with proteins like ATR and Chk1 to coordinate cell cycle arrest and DNA repair linking replication and repair functions to maintain cell stability. It also interacts with the MRN complex (MRE11 RAD50 and NBS1) which is critical for responding to double-strand breaks.
The malfunction of TopBP1 has implications for cancer development and progression. Deficiencies or mutations in TopBP1 may result in impaired DNA repair leading to genome instability a condition frequently observed in cancerous cells. TopBP1 interacts with other tumor suppressors such as p53 to regulate cell cycle checkpoints and prevent the accumulation of mutations. Aberrations in TopBP1 function can also contribute to the pathology of developmental disorders as its interactions with proteins involved in genome maintenance are important for normal cellular function.