Abcam, ab240918, Anti-Acrolein antibody [10A10]

Size: 100µg
Mouse Monoclonal Acrolein antibody. Suitable for IHC, WB, Flow Cyt and reacts with Chemical samples. Cited in 10 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Acrolein.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:10A10,
Isotype:IgG1,
Carrier free:No,
Applications:Flow Cyt, IHC, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Specific for Acrolein modified proteins. Does not detect free acrolein. Does not X-react with Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, MDA, or Methylglyoxal modified proteins.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Acrolein also known as acraldehyde is a highly reactive aldehyde with a molecular weight of 56.06 g/mol. It is a volatile compound commonly found in the environment resulting from combustion processes and as a metabolite of certain drugs and chemicals. At physiological levels acrolein is present in various tissues and cells primarily within the liver where it is generated through lipid peroxidation. It is noteworthy that acrolein does not typically function as a singular enzyme or protein but regulates through its interaction with cellular components.
Biological function summary
Interactions of acrolein with cellular macromolecules such as proteins and DNA occur due to its electrophilic nature. It readily forms adducts leading to structural changes and potentially harmful effects on cellular mechanisms. While it is not part of a larger complex acrolein's formation of adducts significantly alters protein function and gene expression. This reactivity with biological molecules plays an important role in modulating signaling pathways and cellular responses.
Pathways
Interactions involving acrolein affect oxidative stress and inflammation pathways. The compound modifies important proteins like NF-kappaB a transcription factor involved in the immune and inflammation responses. Acrolein's influence on lipid peroxidation links it to the arachidonic acid pathway where it affects processes like prostaglandin synthesis. Associated proteins in these pathways include COX-2 and LOX which play roles in modulating inflammation.
Acrolein's biological interactions have implications in conditions such as cardiovascular disease and cancer. The aldehyde contributes to cardiovascular pathology by damaging endothelial cells and promoting atherosclerosis. Similarly acrolein exposure and its role in DNA damage can facilitate oncogenic processes making it a relevant factor in cancer development. Its connection with proteins like p53 in cancer pathways underlines acrolein's contribution to disease progression and its potential as a target for therapeutic intervention.