Abcam, ab24108, Anti-ABCB4 antibody [P3II-26]

Size: 500µL
Mouse Monoclonal ABCB4 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCB4 aa 600-700.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:P3II-26,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Human,
Applications:Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ABCB4 aa 600-700. The exact immunogen used to generate this antibody is proprietary information.P21439,
Epitope:Clone P3II-26 reacts with an internal epitope of ABCB4.,
Specificity:Clone P3II-26 does not cross-react with the human ABCB1.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-pH: 7.3Preservative: 0.1% Sodium azideConstituents: 0.7% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ABCB4 also known as MDR3 is a member of the ATP-binding cassette (ABC) transporter family with a molecular weight of approximately 140 kDa. This protein is mainly expressed in the canalicular membrane of hepatocytes in the liver. Its primary mechanical function involves the translocation of phosphatidylcholine from the inner to the outer leaflet of the hepatocyte membrane into bile.
Biological function summary
This phospholipid translocation is essential for the formation of bile that is non-toxic and aids in digestion. ABCB4 does not function alone; it operates as a part of a heterocomplex with other proteins that contribute to bile formation and secretion. The absence or dysfunction of this target results in imbalanced bile composition which can negatively impact liver function.
Pathways
The ABCB4 protein plays a significant role in the enterohepatic circulation of bile acids. It interacts closely with the bile salt export pump (BSEP) to facilitate the secretion of bile acids into bile. This protein is also linked to the phospholipid transport exact in the hepatic pathways collaborating with other ABC transporters such as ABCB11 underpinning the finely tuned processes that maintain hepatic homeostasis.
Mutations or deficiencies in ABCB4 are commonly associated with progressive familial intrahepatic cholestasis type 3 (PFIC3) and intrahepatic cholestasis of pregnancy (ICP). Defects in the function of this protein lead to toxic bile accumulation causing cholestasis. Other related proteins such as ABCB11 and ATP8B1 may also influence the occurrence of these disorders due to their overlapping roles in hepatobiliary transport processes.