Abcam, ab241155, Anti-LETM1 antibody

Size: 100µg
Rabbit Polyclonal LETM1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Mitochondrial proton/calcium exchanger protein aa 600-700.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Mitochondrial proton/calcium exchanger protein aa 600-700. The exact immunogen used to generate this antibody is proprietary information.O95202

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibody was affinity purified using an epitope specific to LETM1 immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LETM1 short for Leucine Zipper and EF-Hand Containing Transmembrane Protein 1 plays an important role in cellular ion regulation. This protein with a mass of approximately 81 kDa is mainly located in the inner mitochondrial membrane. LETM1 acts as a mitochondrial calcium and potassium exchanger. It facilitates the transport of calcium out of the mitochondria balancing mitochondrial ion homeostasis. Expression of LETM1 occurs widely across different tissue types indicating its importance in basic cellular function.
Biological function summary
LETM1 contributes to maintaining mitochondrial function and integrity. It comprises part of a complex that includes other mitochondrial membrane proteins. This complex ensures sufficient ion gradients and energy production within the cell. Its role in calcium ion homeostasis aids in regulating mitochondrial membrane potential and ATP synthesis. Efficient operation of this mechanism is essential for cell survival and function.
Pathways
LETM1 integrates into metabolic pathways where it supports mitochondrial bioenergetics and oxidative phosphorylation. It interacts with the OXPHOS system during energy production. LETM1's activity also affects the cellular apoptosis pathway influenced by its regulation of calcium levels. Related proteins like MICU1 and MCU participate in these pathways contributing to the regulation of calcium uptake into mitochondria and further connecting LETM1’s role to broader cellular processes.
LETM1 is linked to Wolf-Hirschhorn syndrome a genetic disorder characterized by developmental delays and seizures. Mutations or deletions affecting LETM1 contribute significantly to the syndrome's phenotype. Additionally LETM1 dysregulation associates with certain types of cancer where aberrant calcium signaling may promote tumor progression. The connection between LETM1 and proteins like Cyclophilin D involved in mitochondrial integrity highlights its influence in disease mechanisms.