Abcam, ab241348, Anti-SLC12A6/KCC3 antibody

Size: 100µg
Rabbit Polyclonal SLC12A6/KCC3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC12A6 aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human SLC12A6 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.Q9UHW9

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibody was affinity purified using an epitope specific to SLC12A6/KCC3 immobilized on solid support, Storage buffer-pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SLC12A6 also known as KCC3 is a potassium-chloride cotransporter involved in regulating ion homeostasis. The protein is big weighing approximately 135 kDa. It moves potassium and chloride ions across cell membranes which affects osmotic balance and cell volume. KCC3 is found widely in the human body with higher expression in the nervous system kidneys and heart. This wide expression suggests its important role in maintaining balance of ions in various tissues.
Biological function summary
KCC3 impacts cellular processes by being an important part of regulating salt balance within cells. It belongs to the cation-chloride cotransporter family and plays an important role in cell volume regulation. KCC3 does not usually function as part of a larger protein complex but acts as a standalone transporter. It helps stabilize cell environment which is necessary for correct cellular signaling and function.
Pathways
KCC3 is integral to maintaining the proper function of the WNK-SPAK/OSR1 kinase pathway. This pathway regulates ion transport and adjusts cell volume after osmotic stress. KCC3 interacts with proteins from this pathway like WNK kinases and SPAK which phosphorylate KCC3 to control its activity. As the transporter modifies ion flow it indirectly affects other signaling pathways dependent on ionic balance within cells.
Mutations in KCC3 link to Hereditary Motor and Sensory Neuropathy with Agenesis of the Corpus Callosum (HMSN/ACC). This disorder affects nerve function indicating KCC3’s role in nervous system health. Additionally KCC3 associates with epilepsy where imbalanced ion homeostasis disrupts neuron function. Related proteins such as WNK kinases when dysregulated have implications for these conditions as well highlighting the complex network KCC3 is involved in.