Abcam, ab241383, Human Fibrinogen ELISA Kit - high sensitivity

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Human Fibrinogen (FGA) ELISA kit is a single-wash, 90-minute SimpleStep ELISA ® used to quantify fibrinogen levels with a sensitivity of 29 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric assay – 450 nm readout, compatible with any standard plate reader - Different formats for different needs – 10 x 96 well plates for bulk orders and 384-well format available for high-throughput applications - Design your own immunoassay: we also offer the conjugation-ready antibody pair in a carrier-free format
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 29 pg/mL,
Range:125 - 8000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Fibrinogen ELISA Kit - high sensitivity ab241383 is a rapid single-wash 90-min ELISA kit to measure Human Fibrinogen in saliva, urine, cell culture supernatant, milk, plasma and serum. This SimpleStep ELISA
sensitivity is 29 pg/mL.
How the assay works
Human Fibrinogen SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human Fibrinogen protein in serum, plasma, urine, saliva, milk, and cell culture supernatant only. Cell and tissue extract samples have not been tested with this kit.
Human Fibrinogen ELISA Kit - high sensitivity ab241383 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Fibrinogen ELISA Kit - high sensitivity ab241383
Human Fibrinogen ELISA Kit - high sensitivity ab241383 has been used to study fibrinogen function and the effects of a novel mutation on fibinogen synthesis, secretion, and polymerization
, extracorporeal bioengineered liver grafts for treating acute liver failure
, and CRSPR/Cas9 to upregulate expression of coagulation factors II, VII, IX, X, and fibrinogen
References:
Fei Chen et al. 2024, PMID: 38233949,
Jeff J Ross et al. 2023, PMID: 37892931,
Mark J Osborn et al. 2022, PMID: 35563479
Save your precious samples
Our 384-well Human Fibrinogen ELISA Kit - high sensitivity only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Fibrinogen Antibody Pair - BSA and Azide free - high sensitivity
ab256605
- Anti-Fibrinogen antibody [EPR19765-189] - BSA and Azide free (Capture)
ab244636
- Anti-Fibrinogen antibody [EPR19765-63] - BSA and Azide free (Detector)
ab244897
Related and recommended products
Human Fibrinogen ELISA Kit - high sensitivity ab241383 is commonly used to study fibrinogen levels in coagulation, fibrinolysis, and liver function.
Other ELISA kits to measure coagulation and liver health include:
- Human AST ELISA Kit (Aspartate Aminotransferase)
ab263881
- Human PAI1 ELISA Kit (SERPINE1)
ab269373
- Human Plasminogen ELISA Kit (PLG)
ab108893

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C