Abcam, ab241581, Anti-Astrin/Deepest antibody

Size: 100µL
Rabbit Polyclonal Astrin/Deepest antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Sperm-associated antigen 5 aa 850-950.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human Sperm-associated antigen 5 aa 850-950. The exact immunogen used to generate this antibody is proprietary information.Q96R06

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-ab241581 was affinity purified using an epitope specific to Astrin/Deepest immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Astrin also known as SPAG5 or Deepest is a protein important for cell division. It has a molecular mass of approximately 45 kDa. Researchers find Astrin primarily in the cytosol and preferably expressed in proliferating cells. Astrin plays a significant role in spindle stabilization during mitosis binding to microtubules to ensure proper chromosome segregation. This protein has become a focus of study due to its critical functions during cell cycle progression.
Biological function summary
Astrin facilitates kinetochore-microtubule attachment participating in the mitotic spindle assembly complex. This complex ensures chromosome alignment and segregation which is critical in cellular replication. Since precise regulation of this process is necessary for genetic stability Astrin's function is indispensable. Its presence influences the structural integrity of dividing cells linking it to the stability of genetic information passed to daughter cells after division.
Pathways
Astrin operates within the spindle assembly checkpoint and cell cycle pathways. The spindle assembly checkpoint pathway ensures that cells do not progress to anaphase until all chromosomes are properly attached to the spindle apparatus. In synergy with proteins like Ndc80 and KNL1 Astrin maintains attachment integrity. Its interactions with these proteins ensure accurate chromosome segregation protecting against chromosomal instability which can lead to aneuploidy.
Astrin's role connects it to conditions like cancer and developmental disorders. Overexpression of Astrin has associations with tumor progression since its function affects cell proliferation. In the context of cancer proteins like Aurora B also influence Astrin's activity. The misregulation of this pathway can lead to faulty chromosomal alignment and segregation contributing to oncogenesis. Such insights make Astrin a potential biomarker and therapeutic target in cancer research.