Abcam, ab241990, Anti-PRIM2 antibody

Size: 100µg
Rabbit Polyclonal PRIM2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PRIM2.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Full Length Protein corresponding to Human PRIM2.P49643

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibody was affinity purified using an epitope specific to PRIM2 Primase Large Subunit immobilized on solid support., Storage buffer-pH: 7 - 8Preservative: 0.09% Sodium azideConstituents: Tris citrate/phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PRIM2 protein also known as DNA primase large subunit plays an essential role in DNA synthesis. With a mass of approximately 58 kDa PRIM2 synthesizes short RNA primers needed for DNA polymerase to start replication. This protein is mainly expressed in tissues with high replication activity including the thymus and bone marrow as well as during the S phase of the cell cycle in various cell types. The PRIM2 protein is critical during the initiation of DNA replication binding to single-stranded DNA to form an RNA-DNA hybrid that DNA polymerases extend.
Biological function summary
DNA primase large subunit forms a complex with the smaller subunit PRIM1 creating the DNA primase enzyme. This complex is part of the larger DNA polymerase alpha-primase complex which is fundamental for the initiation of DNA replication. Primase activity ensures that replication begins accurately and efficiently enabling cells to maintain genomic integrity during cell division. By synthesizing RNA primers PRIM2 facilitates the transition from the initiation to the elongation phase of DNA replication.
Pathways
PRIM2 is a critical component in DNA replication and repair pathways. In the context of DNA replication PRIM2 operates within the framework of the cell cycle pathway particularly in the S phase working closely with other proteins like DNA polymerase alpha PCNA (proliferating cell nuclear antigen) and RFC (replication factor C). PRIM2 also interacts with repair pathways like the NHEJ (non-homologous end joining) pathway ensuring proper genomic transmission across cell divisions by providing primers for DNA polymerases involved in repairing breaks.
Research connects PRIM2 with cancer and genetic instability. Abnormalities in PRIM2 expression or function can lead to replication stress contributing to tumorigenesis. For instance in certain cancers such as leukemia dysregulation of PRIM2 expression alters DNA replication efficiency affecting cell proliferation. Additionally PRIM2 dysfunction can impact the interaction with proteins like p53 an important player in cancer pathways potentially compromising the cell’s ability to respond to DNA damage effectively.