Abcam, ab242115, Anti-VMA21 antibody

Size: 100µL
Rabbit Polyclonal VMA21 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human VMA21 aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human VMA21 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.Q3ZAQ7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Purification notes-Antibody was affinity purified using an epitope specific to VMA21 immobilized on solid support., Storage buffer-pH: 6.8 - 7.4Preservative: 0.09% Sodium azideConstituents: Tris buffered saline, 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VMA21 also known as Vacuolar H+-ATPase assembly factor VMA21 serves an important role in cellular mechanics. This protein with a molecular mass of approximately 9 kDa helps assemble the V-ATPase complex which is important for acidifying vacuoles. VMA21 is expressed in many tissues with higher levels observed in metabolically active tissues due to their need for efficient ion transport and pH regulation.
Biological function summary
VMA21 is an important factor in assembling the V-ATPase complex located in the membranes of vacuoles. The V-ATPase is a proton pump that regulates the pH within organelles by transporting hydrogen ions. VMA21 stabilizes the subunits of V-ATPase allowing its correct function in processes like protein degradation and recycling. This regulation is essential for maintaining a balance in the acidic environment necessary for various cellular processes.
Pathways
VMA21 interacts with the V-ATPase complex impacting the lysosomal acidification and nutrient sensing pathways. It connects with the mTORC1 signaling cascade by affecting lysosomal pH which in turn influences cell growth and metabolism. The protein also has indirect ties with SNARE proteins involved in vesicular transport exemplifying its role in broader biological activities.
VMA21 associates with X-linked Myopathy with Excessive Autophagy (XMEA). Mutations in VMA21 impair the V-ATPase activity leading to deficient lysosomal acidification and autophagy dysregulation. This impairment results in muscle disease characterized by increased autophagic vacuoles. VMA21 also affects other proteins involved in autophagy such as LC3 indicating its extensive role in muscle pathology and metabolic disorders.