Abcam, ab242204, Anti-VGluT1 antibody [CL2754]

Size: 100µL
Mouse Monoclonal VGluT1 antibody. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLC17A7 aa 250-300.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:CL2754,
Isotype:IgG2b,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SLC17A7 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.Q9P2U7,
Epitope:Binds to an epitope located within the peptide sequence EERKYIEDAI as determined by overlapping synthetic peptides.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Purification notes-Purified from TCS., Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
VGluT1 or Vesicular Glutamate Transporter 1 is an important protein for transporting glutamate in the brain. It plays an important role in packaging glutamate into synaptic vesicles. This protein has an approximate mass of 61 kDa. Scientists find VGluT1 expression mainly in the central nervous system especially in areas involved in synaptic transmission and neural communication. VGluT1 localizes at the presynaptic terminals and enables glutamate to be stored in vesicles before release into the synaptic cleft.
Biological function summary
The function of VGluT1 centers on neurotransmitter regulation maintaining adequate synaptic glutamate levels. It does not work alone but works as part of a complex within the presynaptic vesicular organelles. This transport system enables efficient glutamate signaling essential for learning and memory. Through its action it supports important processes in brain development and synaptic plasticity indicating its role in cognitive functions.
Pathways
VGluT1 is essential in the glutamatergic signaling pathways which influence numerous neurotransmission activities. Its activity interlinks with proteins like synaptophysin and synapsins that help dock and release synaptic vesicles. Another associated pathway involves glutamate synthesis and recycling helping maintain excitatory neurotransmission balance. These pathways implicate VGluT1 in maintaining proper neural network connectivity and excitatory-inhibitory balance.
Altered VGluT1 function impacts conditions such as schizophrenia and amyotrophic lateral sclerosis (ALS). Researchers associate its dysregulation with impaired glutamate homeostasis leading to synaptic dysfunction. VGluT1 interacts with neuronal proteins such as EAAT2 involved in ALS where its malfunction results in motor neuron degeneration. In the context of schizophrenia altered VGluT1 expression disturbs glutamatergic neurotransmission contributing to symptoms due to synaptic imbalance.