Abcam, ab24222, Anti-Blood Group H ab antigen antibody [87-N]

Size: 100µg
Mouse Monoclonal Blood Group H ab antigen antibody. Suitable for IP, ELISA, Agg and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:87-N,
Isotype:IgM,
Carrier free:No,
Reacts with:Human,
Applications:Agg, IP, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:The antibody has no cross-reactivity with A or B antigens.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.6Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Blood Group H antigen also recognized as the H/AB antigen plays a significant role in determining blood types alongside A and B antigens. This antigen is part of the ABO blood group system where it acts as a precursor to the A and B antigens. The H antigen is expressed at the cell surface of red blood cells. Its molecular mass can vary as it is part of glycoproteins and glycolipids on the cell membrane. The presence and modification of the H antigen will dictate the ABO blood group of an individual serving as a substrate for specific glycosyltransferases that convert it to A or B antigens.
Biological function summary
The H antigen is important in red blood cell recognition and compatibility in transfusion medicine. It serves as a scaffold for the attachment of specific sugars that form A or B antigens. When present as H/AB in its unaltered form it indicates blood group O. The H antigen is not a part of a complex but interplays with enzymes like the A and B transferases that modify it determining individual blood types. Additionally anti-H antibodies can be found in individuals lacking this antigen like in rare Bombay phenotype emphasizing its importance in blood antigenicity.
Pathways
The H antigen's modification by specific glycosyltransferases is pivotal. These glycosylation pathways dictate the conversion of the H antigen to either the A or B antigens utilizing enzymes encoded by the ABO gene. The enzymatic pathways involving the H antigen relate closely with other proteins like FUT1 and FUT2 which are fucosyltransferases adding fucose to form blood group H. These pathways ensure the diverse presentation of antigens important for immune recognition and compatibility during blood transfusion.
The expression of Blood Group H antigen is linked to transfusion reactions and hemolytic disease of the newborn. Individuals with the rare Bombay phenotype lack the H antigen causing them to produce potent anti-H antibodies leading to complications if transfused with regular O A B or AB blood types. Additionally blood antigens including H/AB have implications in infections and pathology such as when pathogens utilize these antigens as receptors. This highlights the interconnectedness of the H antigen with immune response and cellular recognition processes.