Abcam, ab24223, Anti-Blood Group AB antigen antibody [Z5H-2/Z2A]

Size: 100µg
Mouse Monoclonal Blood Group AB antigen antibody. Suitable for IP, Flow Cyt, ELISA, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:Z5H-2/Z2A,
Isotype:IgM,
Carrier free:No,
Reacts with:Human,
Applications:WB, ELISA, IP, ICC/IF, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.6Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Blood Group AB antigen also known as ABH antigen results from the expression of both A and B antigens on the red blood cell surface. The molecular mass of each antigen depends on the particular oligosaccharide structure. These antigens are expressed on erythrocytes but also found on epithelial and endothelial cells. The presence of both A and B antigens is a unique feature of the AB blood group allowing it to express a combination of glycosyltransferases specifically A and B transferases which modify the H antigen to produce the distinct epitopes of the AB blood group.
Biological function summary
The presence of AB antigens influences blood transfusion compatibility and immune recognition. The antigen does not exist as part of a larger protein complex but instead consists of carbohydrate epitopes attached to lipid or protein backbones. The correct expression of these antigens plays a role in neonatal immune tolerance and can influence responses to organ transplants. These carbohydrate structures are essential for cellular communication and adhesion.
Pathways
The biosynthesis of AB antigens involves glycosylation pathways that include the addition of specific sugar residues. The process begins with the H antigen which the A or B glycosyltransferases then modify and it involves participation from enzymes like fucosyltransferases. The expression and function of these glycosyltransferases also interact with other metabolic pathways related to carbohydrate metabolism. These pathways highlight links with proteins such as α13-N-acetylgalactosaminyltransferase and α13-galactosyltransferase which are responsible for the final antigenic determination.
Blood Group AB antigen holds significance due to its compatibility in blood transfusions and potential risks in organ transplantation. People with AB blood group can receive blood from any group in emergencies due to the lack of anti-A and anti-B antibodies but transplant rejection risks persist due to anti-HLA antibodies. Research connects the expression of AB antigen with certain cancers such as gastric cancer where altered glycosylation patterns could be involved. The antigenic properties can influence interactions with proteins in the immune system such as immunoglobulins and complement proteins potentially modifying disease risk and progression.