Abcam, ab242322, Recombinant human NT-4 protein (Active)

Size: 100µg
Recombinant human NT-4 protein (Active) is a Human Full Length protein, in the 81 to 210 aa range, expressed in Escherichia coli, with >97%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.
Key facts
Purity:>97% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:Escherichia coli,
Tags:Tag free,
Applications:HPLC, FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures is less than 50 ng/ml, corresponding to a specific activity of >2.0 x 104 IU/mg.,
Accession:P34130,
Animal free:No,
Carrier free:Yes,
Species:Human,
Reconstitution:Reconstitute in water or 0.1% BSA aqueous buffer,
Storage buffer:Constituents: PBS

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NT-4 also known as Neurotrophin-4 is a member of the neurotrophin family of proteins. It has a molecular mass of around 14 kDa. NT-4 is expressed in both the central and peripheral nervous systems with significant presence in the brain particularly in regions such as the cortex and hippocampus. This protein acts through binding to the TrkB receptor and plays a direct role in supporting neuronal survival and differentiation.
Biological function summary
NT-4 regulates the growth and maturation of neurons. It is a part of the neurotrophin signaling complex which includes other family members like brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF). By promoting axonal growth and synaptic plasticity NT-4 influences both embryonic neurodevelopment and adult neural adaptations. Its functions are important for maintaining the health of neurons and overall brain function.
Pathways
NT-4 is involved in the neurotrophin signaling pathway and MAPK pathway. It interacts with the TrkB receptor to activate signaling cascades that regulate neuronal survival and plasticity. In these pathways proteins like PIK3CA and MAPK1 interact with NT-4 facilitating its role in signal transduction that influences neural activities and responses to external stimuli.
NT-4 is linked to neurological conditions such as Alzheimer's disease and amyotrophic lateral sclerosis (ALS). In Alzheimer's disease NT-4 in association with BDNF influences neuronal survival and synaptic maintenance which are often compromised as the disease progresses. In ALS interactions between NT-4 and TrkB receptors may affect motor neuron health and survival therefore playing a role in disease pathology. Understanding these relationships can shed light on potential therapeutic strategies.