Abcam, ab24233, Anti-MC4-R antibody

Size: 100µg
Rabbit Polyclonal MC4-R antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 27 publications. Immunogen corresponding to Synthetic Peptide within Mouse Mc4r aa 1-50.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Human,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Mc4r aa 1-50. The exact immunogen used to generate this antibody is proprietary information.P56450

Product details:
This antibody mainly detects an unglycosylated protein at 37kDa and a glycosylated protein at 55kDa.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.4Preservative: 0.02% Sodium azideConstituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The melanocortin-4 receptor also known as MC4-R is a G protein-coupled receptor found within the melanocortin receptor family. It has a molecular mass of approximately 38 kDa. MC4-R is expressed in the central nervous system with high levels in the hypothalamus a region that regulates energy balance and homeostasis. The receptor is activated by several peptide ligands including alpha-melanocyte-stimulating hormone (α-MSH) and it interacts with specific signaling cascades through G proteins.
Biological function summary
The actions through MC4-R are important for energy regulation and appetite control. As part of the melanocortin system MC4-R influences feeding behavior and energy expenditure. Its activation by endogenous or synthetic MC4-R activators suppresses appetite and increases energy consumption. The receptor does not work alone; it forms part of a signaling complex involving other receptor proteins such as MC3-R. The activity of MC4-R also connects to the regulation of body weight and influences fat mass.
Pathways
MC4-R plays an important role in the leptin-melanocortin pathway and the central regulation of energy homeostasis. Leptin a hormone produced by adipose tissues acts upstream by enhancing α-MSH production which in turn activates MC4-R. This activation leads to reduced food intake and increased thermogenesis. Moreover MC4-R interacts with another important signaling protein AGRP (Agouti-related peptide) which acts as an antagonist opposing the effects of α-MSH and regulating energy balance in a complex feedback system.
Mutations or dysregulation of MC4-R are linked to obesity and metabolic syndrome. About 5% of severe early-onset obesity cases involve mutations in the MC4-R gene. The receptor influences the risk for obesity-related conditions through its interactions with AGRP and α-MSH. Additionally compromised MC4-R signaling can lead to disorders such as cachexia where it impacts appetite and metabolism related to conditions like cancer or chronic illness. Defective MC4-R activity therefore is important in understanding the intricate balance between appetite energy expenditure and body mass control.