Abcam, ab242442, Anti-Alpha Dystroglycan antibody [45-3] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal DAG1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:45-3,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab242442 is the carrier-free version of
ab199768
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha dystroglycan (alpha-DG) a subunit of the dystroglycan complex acts mechanically as an important cell surface receptor. This protein binds extracellular matrix components like laminin agrin and perlecan facilitating cell-matrix interactions. Alpha-DG has a molecular weight of approximately 156 kDa and comes from post-translational modification of a precursor protein. Researchers frequently use the terms "alpha" and "DG" when referencing this target. This protein shows wide expression across various tissues including skeletal cardiac and smooth muscle as well as in the brain and peripheral nerves.
Biological function summary
Alpha-DG performs roles critical for stabilizing tissue architecture by connecting the actin cytoskeleton to the extracellular matrix. As a part of the dystroglycan complex alpha-DG associates with beta-dystroglycan contributing to the linkage between dystrophin and the extracellular components. It ensures structural integrity and signal transduction that are necessary for tissue maintenance and function. Disrupted interactions can lead to compromised tissue stability which reflects its importance in cellular contexts.
Pathways
Alpha-DG integrates into the dystrophin-glycoprotein complex (DGC) a significant component within the mechanotransduction and cellular signaling pathways. The complex notably participates in the PI3K/AKT signaling pathway important for survival and metabolism along with interactions involving integrins. Through its role in these pathways alpha-DG engages with proteins like dystrophin and sarcoglycans facilitating proper signaling and structural support which is important for normal muscle function.
Alpha-DG connects closely with dystroglycanopathies which involve a group of muscular dystrophies like congenital muscular dystrophy. These conditions result from improper glycosylation of α-DG affecting its binding ability and compromising muscular integrity. Alpha-DG also relates to conditions such as cancer where altered glycosylation patterns may impact tumor progression and cell invasion. In these contexts alpha-DG interacts with beta-dystroglycan indicating that both defects in glycosylation and associated proteins contribute to disease mechanisms.