Abcam, ab243069, Anti-Sulfotyrosine antibody [12B11]

Size: 100µg
Mouse Monoclonal Sulfotyrosine antibody. Suitable for WB, IHC-P and reacts with Chemical samples. Cited in 1 publication. Immunogen corresponding to Chemical / Small Molecule corresponding to Sulfotyrosine.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:12B11,
Isotype:IgG1,
Carrier free:No,
Applications:IHC-P, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:Specific for sulfated tyrosine residues on various proteins. Does not detect free Sulfotyrosine. Does not cross-react with phosphotyrosine.

Product details:
The clone number has been updated from 7C5 to 12B11, both clone numbers name the same antibody clone.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Purification notes-Purified from TCS., Storage buffer-Preservative: 0.09% Sodium azideConstituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Sulfotyrosine is a post-translational modification of the amino acid tyrosine where a sulfate group gets added to the phenolic hydroxyl moiety. This process is known as tyrosine sulfation and plays a critical role in protein-protein interactions. Tyrosine sulfation occurs primarily in the Golgi apparatus and is mediated by tyrosylprotein sulfotransferases. It doesn't refer to a single protein but rather the chemical modification found on various proteins. The distinct molecular mass for the modification itself is about 80 Da taking into account the addition of the sulfate group. This modification mostly appears in secretory and membrane proteins across a wide range of tissues and organisms.
Biological function summary
The sulfotyrosine modification significantly enhances the binding properties of proteins involved in diverse biological processes. It affects proteins such as adhesion molecules G protein-coupled receptors and coagulation factors often being essential for their proper function. Many proteins containing sulfotyrosine participate in forming multi-protein complexes that mediate cell signaling and communication.
Pathways
Sulfotyrosine modifications integrate notably into signal transduction and immune response pathways. Proteins like P-selectin glycoprotein ligand-1 (PSGL-1) and chemokine receptors such as CCR5 require sulfotyrosine for optimal receptor-ligand interactions which impacts leukocyte trafficking and chemotaxis. These modifications often serve as docking sites for other proteins coordinating complex pathways important for cellular responses.
Sulfotyrosine modifications have implications in inflammatory diseases and cancer. The modification plays a role in mediating the interactions involving proteins like PSGL-1 in immune response pathways which are critical in inflammatory diseases such as rheumatoid arthritis. Additionally abnormal tyrosine sulfation on chemokine receptors like CCR5 has associations with the metastatic properties of cancer influencing both tumor development and immune evasion mechanisms. Their correlation emphasizes the importance of understanding sulfotyrosine's functional roles in disease pathogenesis.