Abcam, ab243106, Anti-Collagenase ColA antibody [cp-02]

Size: 100µg
Mouse Monoclonal COLA antibody. Suitable for WB and reacts with Clostridium perfringens, Clostridium histolyticum samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:cp-02,
Isotype:IgM,
Carrier free:No,
Reacts with:Clostridium perfringens, Clostridium histolyticum,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Specificity:with collagenases of Clostridium perfringens and C.histolyticcum.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified using Ab-Capture for IgM., Storage buffer-pH: 6 - 8.5Constituents: PBS, 50% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Collagenase ColA also known as Collagenase G is an enzyme that breaks down collagen in the extracellular matrix. This enzyme belongs to the family of metalloproteases and has a mass of approximately 116 kDa. Collagenase ColA is expressed in a variety of bacteria notably in Clostridium histolyticum. It plays an important role in tissue remodeling and bacterial invasion by facilitating the breakdown of collagen which is the most abundant protein in the human body.
Biological function summary
Collagenase ColA enables bacterial pathogens to penetrate host tissues by degrading structural barriers composed of collagen. It is not part of a large complex but functions individually to exert its collagen-degrading action. This ability to degrade collagen makes it essential for bacterial infection processes and host tissue infiltration highlighting its significance in microbial pathogenicity.
Pathways
Collagen degradation involves the participation of Collagenase ColA impacting processes like connective tissue breakdown and remodeling. It collaborates with other proteases and is involved in extracellular matrix pathways. The action of this enzyme also links with protease-activated receptor signaling pathways which can involve other related proteins such as gelatinases and stromelysins that share collagenolytic activities.
Increased activity of Collagenase ColA is associated with diseases like necrotizing fasciitis and bacterial infections leading to cellulitis. These disorders often result due to the enzyme's capability to assist bacterial spread by degrading host tissue barriers. Moreover increased collagenase activity can link to other proteins like matrix metalloproteinases which are often upregulated in inflammatory conditions and tissue degradation diseases.