Abcam, ab243482, Anti-SHLD1 antibody

Size: 100µL
Rabbit Polyclonal SHLD1 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SHLD1 aa 50-150.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SHLD1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.Q8IYI0

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SHLD1 also known as shieldin complex subunit 1 functions as part of the shieldin complex involved in DNA repair. SHLD1 has a molecular mass of approximately 22 kDa. It is expressed in various tissues and is most abundant in cells that are actively dividing such as those found in cancer cell lines. SHLD1 plays a mechanical role by acting as a scaffold protein that contributes to the non-homologous end joining (NHEJ) pathway of DNA double-strand break repair.
Biological function summary
SHLD1 participates as a component of the shieldin complex alongside SHLD2 SHLD3 and REV7. Together these proteins facilitate the repair of DNA double-strand breaks by directing 53BP1-mediated NHEJ. This complex stabilizes the DNA ends and assists in their processing which prevents excessive resection and promotes the proper end-joining process. SHLD1's role contributes significantly to maintaining genomic integrity.
Pathways
SHLD1 integrates within the DNA damage response specifically the NHEJ pathway. This pathway is important for fixing double-strand breaks and involves several other proteins like KU70 KU80 DNA-PKcs and 53BP1. SHLD1 supports the action of 53BP1 ensuring its recruitment to DNA damage sites which helps initiate and regulate the repair process.
SHLD1's involvement in genomic stability links it to cancer and immunodeficiency syndromes. Aberrations in the NHEJ pathway influenced by SHLD1 dysfunction can lead to tumorigenesis as improper DNA repair may trigger mutations and chromosomal rearrangements. SHLD1 also relates to proteins like BRCA1 which is known for its role in homologous recombination repair pathways. Understanding SHLD1 function has potential implications in developing treatments that enhance DNA repair capacity in disease contexts.