Abcam, ab243667, Anti-ATP8 antibody [1G3-1H11-1C3]

Size: 100µg
Mouse Monoclonal ATP8 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MT-ATP8.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:1G3-1H11-1C3,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Human MT-ATP8. The exact immunogen used to generate this antibody is proprietary information.P03928

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein L, Purification notes-Protein L affinity chromatography from Fetal Bovine Serum-containing medium. Purity: Near homogeneity as assessed by SDS-PAGE and Coomasie Blue staining., Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: HEPES, 0.87% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP8 also known as MT-ATP8 is a mitochondrial protein encoded by the mitochondrial DNA. It functions as a part of the ATP synthase complex or complex V of the electron transport chain. This complex is responsible for ATP production through oxidative phosphorylation. ATP8 is a small protein with a molecular mass of approximately 10 kDa. It is highly expressed in tissues with high energy demand such as the heart liver and muscle.
Biological function summary
The protein plays an important role in maintaining the mitochondrial membrane potential and ATP synthesis. ATP8 is a component of the F0 sector of the ATP synthase complex which is important for proton translocation across the mitochondrial inner membrane. This translocating activity is essential for the synthesis of ATP from ADP and inorganic phosphate. As part of this complex ATP8 contributes to cellular energy metabolism and regulation.
Pathways
ATP8 is involved in the oxidative phosphorylation pathway and plays an integral role in energy metabolism. It works within the ATP synthase enzyme complex which collaborates with other complexes in the electron transport chain to produce ATP. Related proteins in this pathway include ATP6 and COX1 both important for efficient electron transfer and energy production.
ATP8 mutations or dysfunctions can relate to mitochondrial disorders such as Leigh syndrome and mitochondrial myopathy. These conditions arise from defects in mitochondrial energy production. The protein's interactions with ATP6 further highlight the interconnected nature of mitochondrial disorders as both proteins are involved in maintaining ATP synthase functionality. Understanding ATP8's role in these diseases helps in uncovering potential therapeutic targets.