Abcam, ab243690, Human DKK4 ELISA Kit

Size: 1 x 96Tests
Human DKK4 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human DKK4 with a sensitivity of 5.17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 5.17 pg/mL,
Range:21.88 - 1400 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human DKK4 ELISA Kit (ab243690) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DKK4 protein in cell culture supernatant, cit plasma, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DKK4 with 5.17 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (
ab203359
) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Dickkopf-related protein 4 (DKK4) acts as an inhibitor of the Wnt signaling pathway which affects cell fate proliferation and migration. Known aliases for DKK4 include DKK-4 and DKK-4 protein. It possesses a molecular mass of around 27 kDa. DKK4 mainly expresses in the skin gastrointestinal tract and placenta indicating roles in these tissues and systems. Its presence suggests involvement in processes related to epithelial and stromal interactions.
Biological function summary
DKK4 participates in the regulation of embryonic development and tissue homeostasis. It functions as a part of the larger Dickkopf protein family which modulates Wnt signaling by binding to LRP5/6 co-receptors. Through this interaction DKK4 manages the balance between cell growth and apoptosis important in both early development and adult tissue maintenance. DKK4 presence is often necessary to modulate signals that govern differentiation and proliferation in complex tissue systems.
Pathways
DKK4 predominantly interacts within the Wnt signaling pathway. This pathway is critical for regulating gene expression involved in stem cell maintenance and differentiation. DKK4 acts by interacting with proteins such as LRP6 and Kremen thereby inhibiting Wnt-induced processes. Through modulation of these factors DKK4 ensures the fine-tuning of signaling cascades necessary for maintaining cellular equilibrium under physiological conditions.
Research associates DKK4 with malignant melanoma and colorectal cancer. These associations arise due to DKK4's role in modulating Wnt signaling which can drive oncogenic processes if dysregulated. In melanoma DKK4 can affect tumor cell proliferation by interacting with proteins like beta-catenin. In colorectal cancer abnormal expression of DKK4 might disrupt normal signaling balance further implicating it in tumor growth and progression. These connections highlight DKK4's potential as both a biomarker and a therapeutic target.