Abcam, ab244347, Anti-SPEN antibody

Size: 100µL
Rabbit Polyclonal SPEN antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human SPEN aa 100-300.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human SPEN aa 100-300. The exact immunogen used to generate this antibody is proprietary information.Q96T58

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPEN also known as Split Ends or SHARP is a large nuclear protein with a mass of approximately 400 kDa. It is expressed in various tissues including the brain heart and lungs. Mechanically SPEN functions as an RNA-binding protein with C-terminal SPOC (Spen Paralog and Ortholog C-terminal) domains which allow it to interact with other cellular components. These interactions regulate transcription by modulating the activity of coactivators and corepressors at specific gene sites.
Biological function summary
SPEN plays a role in transcriptional repression and gene silencing by being part of a multiprotein complex. It interacts with nuclear receptors and RNA polymerase II inhibiting the transcriptional activity of target genes. SPEN helps regulate critical cellular processes such as differentiation and proliferation. It acts as a transcriptional corepressor by interacting with nucleic acids and influencing chromatin structure impacting gene expression in a context-dependent manner.
Pathways
SPEN holds an important role in the Notch signaling pathway. This pathway is important for cell fate determination and tissue homeostasis. SPEN also interacts with the estrogen receptor signaling pathway where it can influence the transcriptional outcome. Through these pathways SPEN associates with proteins such as RBPJ a transcriptional regulator in the Notch pathway and ERα (estrogen receptor alpha) a vital component in the estrogen signaling pathway.
SPEN has connections to certain cancers particularly breast cancer and neurological conditions like autism spectrum disorders. In breast cancer SPEN can interact with ERα influencing the proliferation of cancer cells through estrogen receptor signaling. In neurological conditions dysregulation of SPEN's interaction with proteins involved in neural development might contribute to disease mechanisms. Understanding these associations helps in potential therapeutic targeting of SPEN in relevant diseases.